8种市售金银花露饮料的挥发性化合物测定分析

Determination and analysis of volatile compounds in 8 kinds of commercial honeysuckle beverages

目的比较分析市售金银花露饮料的挥发性化合物。方法采用顶空固相微萃取-气相色谱质谱联用(headspace-solid phase microextraction-gas chromatography-mass spectrometry,HS-SPME-GC-MS)技术测定8种金银花露饮料,结合含量分析和主成分分析讨论异同。结果共鉴定出129个化合物,包含萜类、醛类、醇类、杂环、酮类、酯类等物质。半定量分析结果表明:8种产品的挥发性成分总量相差较大,范围覆盖11.98~76.61μg/g。在8种产品中都有检出的化合物共33个,主要包括芳樟醇、2-乙基-1-己醇、α-松油醇、糠醛、苯甲醛、香叶醇等,仅在某1种产品中检出的化合物共29个,主要包括3-戊烯-2-酮、乙酸薄荷酯、异蒲勒醇、肉桂醛等。主成分分析提取了4个主成分累计贡献率达87.00%,其中前3个主成分综合的主要分别是醛类、芳香族、萜类物质,说明这些物质可以作为衡量金银花露饮料品质的重要指标。结论为金银花露生产企业把控产品品质提供了相关理论依据。

Objective To compare and analyze the volatile compounds in the commercial honeysuckle beverages. Methods The volatile components of eight honeysuckle beverages were determined by headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS). The similarities and differences of eight products was discussed by relative content analysis and principal component analysis. Results A total of 129 compounds,including terpenes,aldehydes,alcohols,heterocycles,ketones,esters,etc.,were identified. The results of semi-quantitative analysis showed that the total volatile components of the eight products varied greatly,ranging from 11.98 μg/g to 76.61 μg/g. 33 compounds,including linalool,2-ethyl-1-hexyl alcohol,α-terpineol,furfural,benzaldehyde,vanilla alcohol,etc.,were identified in all the eight products. 29 compounds,including 3-pentene-2-one,menthyl acetate,isopulegol,cinnamaldehyde,etc.,were identified in only one product. The cumulative contribution rate of 4 principal components was 87.00% by principal component analysis,and the first three principal components mainly integrated aldehyde,aromatic and terpene,which may serve as important indicators to measure the quality of honeysuckle beverages. Conclusion This study provided a theoretical basis for the manufacturers to control the quality of the product.