分光光度法检测葡萄酒中花青素方法可行性分析

Feasibility analysis for the determination of anthocyanin in wine by spectrophotometry

目的分析分光光度法检测葡萄酒中花青素方法的可行性,并对方法进行优化。方法通过分析标准品和样品的全波长扫描图,确定分光光度法的最佳检测波长;通过检测不同储存时间时标准品吸光值,分析标准品稳定性,确定标准品储备液最佳使用时间;通过检测加标回收率、检出限、定量限等参数,同时与液相色谱法的数据作对比,分析该方法的稳定性和准确性。结果最佳检测波长为518nm;标准品母液在2d内吸光值比较稳定;线性范围为1~20μg/mL时,r^2为0.9998,检出限为0.188 mg/100 mL,定量限为0.625 mg/100 mL。加标水平为4、8、12 mg/100 mL时,加标回收率为102.3%~106.5%。分光光度法检测葡萄酒样品中花青素含量为(6.58±0.38) mg/100 mL,液相色谱法检测样品中总花青素含量为(6.84±0.021) mg/100 mL。结论分光光度法适合用于葡萄酒中花青素含量的检测,方法准确性较好。

Objective To analyze the feasibility of spectrophotometric determination of anthocyanins in wine and optimize the method. Methods The best detection wavelength of spectrophotometry was optimized by analyzing the scanning diagram of the standard sample with full wavelength. By detecting the absorbance value of the standard at different storage time,the stability of the standard was analyzed,and the optimal storage time of the standard reserve liquid was analyzed. By detecting standard recovery,detection limit and quantitative limit,and comparing with the data of liquid chromatography,the stability and accuracy of the method were analyzed. Results The best detection wavelength was 518 nm. The absorbance of standard solution was stable within 2 d. When the linear range was 1-20 μg/mL,r2 was 0.9998,the limit of detection was 0.188 mg/100 mL,and the limit of quantification was 0.625 mg/100 mL. The recoveries were 102.3%-106.5% when the spiked levels were 4,8 and 12 mg/100 mL. The content of anthocyanin in wine samples was(6.58±0.38) mg/100 mL by spectrophotometry and(6.84±0.021) mg/100 mL by liquid chromatography. Conclusion Spectrophotometry is suitable for the determination of anthocyanin content in wine with high accuracy.