穴位电针对慢性神经病理性疼痛大鼠脊髓前列腺酸性磷酸酶表达的影响

Effect of electroacupuncture of points on prostatic acid phosphatase expression in spinal cord of rats with chronic neuropathic pain

目的观察电针对神经病理性痛大鼠脊髓前列腺酸性磷酸酶(PAP)的表达,及腺苷含量及大鼠痛阈变化的影响,探讨电针镇痛机制。方法21只雄性SD大鼠采用慢性坐骨神经压缩模型(CCI),取“足三里”及“太冲”穴。设假手术组(sham组)模型组(CCI组)和电针治疗组(EA组)。采用行为学检测大鼠术前、术后第7天和电针治疗后大鼠机械痛敏值和热敏痛阈值变化,采用Real-time PCR技术检测脊髓PAP mRNA的表达,高效液相色谱法检测脊髓腺苷含量。结果相比CCI组大鼠,电针组大鼠机械痛敏值和热敏痛阈值明显提高(P<0.05,P<0.05)。电针组大鼠脊髓PAP mRNA表达和腺苷含量比CCI组大鼠显著增加(P<0.05)。与CCI组大鼠相比,电针组大鼠脊髓腺苷含量增加(P<0.01)。结论电针可提高神经病理性痛大鼠机械痛阈值和热敏痛阈值,其机制可能是通过增加脊髓PAP mRNA的表达和腺苷含量发挥作用。

Objective To observe the effect of electroacupuncture on the expression of spinal prostatic acid phosphatase( PAP) mRNA and adenosine concentration in neuropathic pain rats. Methods Twenty-one male adult SD rats were randomized into sham operation group( sham group),chronic constrictive injury group( CCI group) and electroacupuncture treatment group( EA group). Electro acupuncture treatment was applied to Tsusanli( ST36) and Taichung( LR3) points. The mechanical pain threshold and thermal pain threshold were measured by using electronic von frey anesthesiometer and rediant-heat irradiation separately on pre-CCI 1 day,post-CCI 7 days and post-EA treatment.Real-time PCR was applied to measure the expression of spinal cord PAP mRNA. High performance liquid chromatography( HPLC) was applied to measure spinal cord adenosine concentration. Results Compared with the CCI group rats,mechanical and thermal pain thresholds in EA group rats were increased significantly( P < 0. 05,P < 0. 05). The expression of PAP mRNA in the spinal cord in EA group rats was increased significantly compared with the CCI group rats( P <0. 05). Simultaneously,the extracellular concentration of spinal adenosine in EA group rats was increased compared with the CCI group rats( P < 0. 01). Conclusion It demonstrated electroacupuncture can improve mechanical and thermal pain thresholds in neuropathic pain rats,and PAP and adenosine may be involved into the analgesia mechanism in spinal cord.