一种新的乳腺癌预后标志物--着丝粒蛋白M

Centromere protein M:a novel biomarker for prognosis of human breast cancer

目的着丝粒蛋白M(CENP-M)是组成型着丝粒相关网络(CCAN)蛋白家族的一名重要成员,它可以保证着丝粒、动粒的正确形成和功能行使,在细胞正常分裂中发挥重要作用。但是目前有关CENP-M与癌症的研究很少。因此,本实验旨在探讨CENP-M的表达水平与乳腺癌预后之间的关系。方法利用Oncomine数据库、COSMIC数据库、bc-GenExMiner、Kaplan-Meier plotter、cBio Portal、GEPIA和Timer数据库系统,分析CENP-M的表达和乳腺癌预后情况。结果与正常组织相比,CENP-M在乳腺癌组织(尤其在侵袭性导管乳腺癌、侵袭性筛状乳腺癌和黏液性乳腺癌)中表达上调;基因突变分析表明,CENP-M表达增加不是由基因序列或拷贝数的改变引起的;CENP-M的表达和乳腺癌不同分型以及Scarff-Bloom-Richarolsn(SBR)分级相关;CENP-M高表达的乳腺癌患者预后较差;乳腺癌中CENP-M的表达和CDC45的表达相关。结论 CENP-M可以作为乳腺癌患者的预后标志物。

Objective Centromere protein M( CENP-M) is an important member of constitutive centromereassociated network( CCAN) protein family. And it guarantees the correct formation and function of centromeres and kinetochores,and plays an important role in normal cell division. However,there are only a few studies on the relationship between CENP-M and cancers. Therefore,the aim of this study is to explore the relationship between CENP-M expression and prognosis of breast cancer. Methods CENP-M expression and prognosis in breast cancer were analyzed through datebases,including Oncomine,COSMIC,bc-GenExMiner,Kaplan-Meier plotter,c Bio Portal,GEPIA and Timer. Results Compared with the normal tissue samples,the expression of CENP-M was up-regulated in various cancers,and the highest expression was found in breast cancer( especially in invasive ductal breast cancer,invasive cribriform breast adenocarcinoma and mucinous breast carcinom);According to analysis of genic mutation,the increase of CENP-M expression was not due to the change of the sequence or copy number of the gene;The expression of CENP-M was correlated with different types and Scarff-Blom-Richordson( SBR) stages of breast cancer;Breast cancer patients with high CENP-M expression had poor prognosis;The expression of CENP-M was correlated with the expression of CDC45 in breast cancer. Conclusion CENP-M can be a prognostic biomarker for breast cancer patients.