组织蛋白酶C和肿瘤坏死因子α在人冠脉组织中的表达及其与冠心病的相关性研究

Expression of cathepsin C and tumor necrosis factor-αin human coronary artery and their relationships with coronary heart disease

目的:检测组织蛋白酶C(CTSC)和肿瘤坏死因子α(TNF-α)在人冠状动脉组织中的表达,并探讨两者在冠心病中的可能作用机制。方法:收集贵州医科大学法医司法鉴定中心2018年10月~2019年12月的52例冠心病死亡案例的冠状动脉作为冠心病组,以同期25例无任何心脏疾病的意外死亡案例作为对照组。通过病理组织学检查冠状动脉管腔狭窄情况和血管内膜斑块形成情况;采用Western blot法检测CTSC和TNF-α的蛋白表达;免疫组织化学染色方法检测CTSC及TNF-α在细胞内的表达及定位。结果:HE染色见对照组冠状动脉内膜光滑平整、无增厚,管腔未见狭窄;冠心病组中血管内膜不规则增厚、粥样硬化斑块形成,中膜明显受压变薄,管腔呈不同程度偏心性狭窄(P<0.05)。Western blot结果显示,与对照组相比,CTSC和TNF-α在冠心病组中的表达显著升高(P<0.05)。免疫组织化学染色显示,CTSC和TNF-α均在泡沫细胞胞质中表达,且其阳性表达均显著高于对照组(P<0.05)。Pearson积矩相关分析结果显示,CTSC和TNF-α在冠心病中的表达呈正相关(r2=0.743,P<0.05)。结论:CTSC在冠状动脉组织中的表达上调可能促进了TNF-α的表达,从而影响冠心病的发生发展。

AIM:To explore the possible mechanism of cathepsin C(CTSC)and tumor necrosis factor-α(TNF-α)in coronary heart disease(CHD)by detecting the protein expression of CTSC and TNF-αin human coronary artery tissue.METHODS:The coronary artery tissues from 52 cases of CHD and 25 cases of accidental death without any heart disease in the Forensic Judicial Expertise Center of Guizhou Medical University from October 2018 to December 2019 were collected as CHD group and control group,respectively.The coronary artery stenosis and intimal plaque formation were examined by histopathology,the protein expression of CTSC and TNF-αwas determined by Western blot,and the intracellular expression of CTSC and TNF-αwas analyzed by immunohistochemical staining.RESULTS:The results of HE staining showed that the intima of coronary artery in control group was smooth,and no thickening or stenosis was observed.In CHD group,the intima thickened irregularly,atherosclerotic plaques formed,the intima became thinner and the lumen showed eccentric stenosis in varying degrees(P<0.05).The results of Western blot showed that the expression of CTSC and TNF-αin CHD group was significantly higher than that in control group(P<0.05).Immunohistochemical staining showed that both CTSC and TNF-αwere expressed in the cytoplasm of foam cells,and their positive expression was significantly higher than that in control group(P<0.05).The results of Pearson moment correlation analysis showed that there was positive correlation between the expression of CTSC and TNF-αin CHD(r2=0.743,P<0.05).CONCLUSION:The up-regulated expression of CTSC in coronary artery tissue may promote the expression of TNF-αand affect the occurrence and development of CHD.