黄芪甲苷对多囊卵巢综合征大鼠性激素水平及氧化应激损伤的影响

Effects of astragaloside on the levels of sex hormone and oxidative stress injury in rats with polycystic ovary syndrome

目的:探讨黄芪甲苷对多囊卵巢综合征(PCOS)大鼠性激素水平及氧化应激损伤的影响。方法:将60只雌性SD大鼠随机分成正常对照组、模型组、阳性对照达英-35(0.3392 mg/kg)组、黄芪甲苷低剂量(12.5 mg/kg)组和黄芪甲苷高剂量(50 mg/kg)组,每组12只。采用来曲唑(1 mg/kg)灌胃诱导PCOS模型,造模成功后灌胃给药,每天1次,连续3周。给药结束后,通过阴道涂片观察各组大鼠动情周期,计算卵巢指数,HE染色观察卵巢组织病理学变化,ELISA法检测血清性激素睾酮(T)、雌二醇(E2)、促黄体生成素(LH)和卵泡刺激素(FSH)水平,比色法检测血清及卵巢组织中超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)水平,Western blot检测卵巢组织中甾类激素合成急性调节蛋白(StAR)及凋亡相关蛋白cleaved caspase-3、Bax和Bcl-2表达水平。结果:与正常对照组比较,模型组大鼠动情周期紊乱,卵巢指数增加,卵巢呈多囊样改变,血清中T、LH和MDA含量显著增加(P<0.05),E2和FSH含量及GSH-Px和SOD活性显著降低(P<0.05),卵巢组织中MDA含量及StAR、cleaved caspase-3和Bax蛋白水平显著上调(P<0.05),GSH-Px和SOD活性及Bcl-2蛋白水平显著下调(P<0.05);与模型组比较,黄芪甲苷高剂量组和达英-35组大鼠动情周期恢复,卵巢指数降低,卵巢多囊样明显减少,血清中T、LH和MDA含量显著减少(P<0.05),E2和FSH含量以及GSH-Px和SOD活性显著增加(P<0.05),卵巢组织中MDA含量及StAR、cleaved caspase-3和Bax蛋白水平显著下调(P<0.05),GSH-Px和SOD活性及Bcl-2蛋白水平显著上调(P<0.05),而黄芪甲苷低剂量组无显著变化(P>0.05)。结论:黄芪甲苷能有效平衡PCOS大鼠的性激素水平,缓解机体氧化应激损伤,其机制可能与抑制StAR的表达有关。

AIM:To investigate the effects of astragaloside on the levels of sex hormone and oxidative stress in rats with polycystic ovary syndrome(PCOS).METHODS:Female SD rats(n=60)were randomly divided into normal control group,model group,Diane-35(0.3392 mg/kg)group,low dose astragaloside(12.5 mg/kg)group and high dose astragaloside(50 mg/kg)group,with 12 rats in each group.The PCOS model was induced by letrozole(1 mg/kg),which was administered by gavage once a day for 3 weeks.After administration,the estrus cycle of the rats was observed by vaginal smear,and the ovarian index was calculated.HE staining was used to observe the histopathological changes of the ovaries.Serum levels of the sex hormones testosterone(T),estradiol(E2),luteinizing hormone(LH)and follicle-stimulating hormone(FSH)were measured by ELISA.The levels of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)in serum and ovarian tissue were detected by colorimetry,and the protein levels of steroidogenetic acute regulatory protein(StAR)and apoptosis-related proteins cleaved caspase-3,Bax and Bcl-2 in ovarian tissue were detected by Western blot.RESULT:Compared with control group,the oestrous cycle of the rats in model group was disorder,and the ovarian index was increased,ovary was polycystic.The serum levels of T,LH and MDAwere significantly increased(P<0.05),while the contents of E2,FSH and the activities of GSH-Px and SOD were significantly decreased(P<0.05).The levels of MDA,StAR,cleaved caspase-3 and Bax proteins in ovarian tissue were significantly up-regulated(P<0.05).GSH-Px and SOD activities and Bcl-2 protein levels were significantly down-regulated(P<0.05).CON⁃CLUSION:Astragalosideeffectively balances the levels of sex hormone in PCOS rats and relieves the oxidative stress injury,the mechanism may be related to the inhibition of StAR expression.