糖尿病肾病患者血清中性粒细胞明胶酶相关脂质运载蛋白含量检测及其与肾功能损伤、肾纤维化的相关性分析

Detection of serum NGAL in diabetic nephropathy patients and its correlation with renal dysfunction and fibrosis

目的检测糖尿病肾病(DN)患者血清中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的含量,评估NGAL含量与患者肾功能损伤及肾纤维化程度的相关关系。方法 100例DN患者根据UAER水平分为DN前期组(UAER <30 mg/24 h)28例、DN早期组(30 mg/24 h≤UAER≤300mg/24 h)52例、DN临床组(UAER> 300 mg/24 h)20例。取同期在院进行体检的健康志愿者50例作为正常对照组。对比四组研究对象血清NGAL含量及肾功能[血肌酐(SCr)、尿素氮(BUN)、血尿酸(BUA)]、肾纤维化指标[层粘连蛋白(LN)、透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)]水平的差异,采用Pearson检验评估DN患者血清NGAL含量与肾功能损伤、肾纤维化的相关关系。结果 DN患者随病情加重,血清NGAL含量增加,肾功能指标SCr、BUN、BUA及肾纤维化指标LN、HA、PCⅢ、Ⅳ-C的水平也增加(P <0.05)。相关性分析发现,DN患者血清NGAL含量与肾功能指标、肾纤维化指标水平均呈正相关关系(P <0.05)。结论 DN患者血清NGAL含量异常上升,具体含量与机体肾损伤程度、肾纤维化程度直接相关,可作为评估DN病情的可靠指标之一。

Objective To detect serum content of NGAL in diabetic nephropathy(DN)patients,and evaluate the correlation between NGAL and degree of renal damage and fibrosis.Methods 100 cases of DN patients in our hospital were divided into pre-DN group(UAER<30 mg/24 h),early DN group(30 mg/24 h≤UAER≤300 mg/24 h)and clinical DN group(UAER>300 mg/24 h).50 healthy volunteers at the same period were selected as normal control group.Serum content of NGAL,renal function and renal fibrosis were compared among four groups.Pearson test was used to evaluate the relationship between serum content of NGAL and renal function injury,renal fibrosis in DN patients.Results As aggravation of DN,serum content of NGAL increased,and the levels of SCr,BUN,BUA,LN,HA,PC III,IV-C were also increased(P<0.05).Correlation analysis showed that serum NGAL level was positively correlated with renal function index and renal fibrosis index in DN patients(P<0.05).Conclusion Serum content of NGAL in DN patients increases abnormally,and the specific content is directly related to the degree of kidney injury and renal fibrosis.It can be used as a reliable index to evaluate the condition of DN.