玉米赤霉烯酮对小鼠B淋巴细胞体外活化增殖及分化的影响

Effects of zearalenone on the activation,proliferation and differentiation of mice B lymphocytes in vitro

为了探究玉米赤霉烯酮(zearalenone,ZEA)对离体B淋巴细胞活化、增殖及分化的影响,试验以BALB/c小鼠脾脏B淋巴细胞为材料,分设细胞空白对照组(Control)、脂多糖对照组(LPS 5?g/m L)、不同浓度ZEA染毒组(LPS+10、20或30?mol/L ZEA),CCK-8法检测24 h时细胞相对活性,流式细胞术分别检测6 h、72 h时淋巴细胞活化标识分子CD69、CD71的表达量,以及72 h时浆细胞表面标识分子CD138的表达量,CFSE法检测B淋巴细胞的增殖情况。结果显示,ZEA在终浓度10、20、30?mol/L下均可显著或极显著地降低LPS诱导的B细胞相对活性,并导致细胞表面标识分子CD69、CD71、CD138的表达量下降(P<0.05或P<0.01)。此外,ZEA还可抑制B细胞的增殖,且呈剂量效应关系。结论认为,玉米赤霉烯酮可直接通过抑制小鼠B淋巴细胞的活化、增殖、分化,影响体液免疫的进程,从而对动物机体产生免疫抑制作用。

To investigate the function of zearalenone(ZEA)on the activation,proliferation and differentiation of mice B lymphocytes in vitro,the experiment used the BALB/c mice B lymphocytes as the material,and set up the cell blank group(Control),LPS relative group(LPS 5?g/mL)and different concentrations of ZEA exposure group(LPS+10,20 or 30?mol/L ZEA).The relative activity of B lymphocytes were detected by CCK-8 method at 24 h.The expressions of CD69 and CD71 were detected respectively by flow cytometry at 6 h and 72 h.The expression of CD138 which is the typical surface marker of plasma cell,was also detected by flow cytometry at 72 h.The proliferation of B cell was detected by CFSE method.The results showed that ZEA reduced the relative activity of LPS-mediated B cells and the expression of CD69,CD71 and CD138 in B cells at a final concentration of 10,20,30?mol/L(P<0.05 or P<0.01).It also inhibited the proliferation of B cell,which showed a dose-effect relationship.In conclusion,ZEA can directly inhibit the activation,proliferation and differentiation of mice B lymphocytes in vitro.It can also affect the whole progression of humoral immune,which can cause immunosuppression in animals.