地塞米松对刺鼠基因相关蛋白神经元和阿片促黑素皮质素原神经元功能活性的影响

Effects of dexamethasone on the functional activity of AgRP and POMC neurons

为探讨应激状态下下丘脑食欲相关神经元功能活性的变化,本研究选用小鼠下丘脑N38(mHypoE-N38)细胞系和阿片促黑素皮质素原(POMC)细胞系为研究对象,采用长效的糖皮质激素地塞米松(DXMS)进行急性处理,利用CCK-8检测试剂盒、细胞免疫荧光、荧光定量PCR(qPCR)和Western blot等技术对细胞活力及功能进行了检测。研究结果显示:DXMS对POMC细胞的兴奋性和分泌POMC的功能均无显著影响,而对于N38细胞,400 ng/mL和700 ng/mL的DXMS均能显著抑制细胞的兴奋性,降低N38细胞的刺鼠基因相关蛋白(AgRP)表达及分泌,且N38细胞糖皮质激素受体(GR)的表达显著下降,细胞活力显著降低。研究表明,地塞米松能影响促进食欲的AgRP神经元,抑制其兴奋性,降低细胞活力,并且抑制AgRP的表达及分泌,而对抑制食欲的神经元POMC功能无显著影响。

In order to study variation in the functional activity of hypothalamic appetite-related neurons in the stressed state,the mouse hypothalamus N38(mHypoE-N38)cell line and the opioid adrenocorticotropic hormone(POMC)cell line were used as subjects in this research.Dexamethasone(DXMS)as a long-acting glucocorticoid was used in the designed acute treatment.The viability and function of the cells were tested using a CCK-8 detection kit,cell immunofluorescence,quantitative PCR and Western bolt.The results showed that DXMS had no significant effect on the excitability of the POMC cells and on the function of the secreting POMC.For the N38 cells,400 ng/mL and 700 ng/mL of DXMS significantly inhibited the excitability of the cells and reduced their AgRP.In addition to expression and secretion,GR expression was significantly increased in the N38 cells,and the viability of the cells was significantly reduced.In short,dexamethasone mainly influenced the appetite-promoting AgRP neurons in the hypothalamus,suppressing their excitability,reducing cell viability,and inhibiting the expression and secretion of AgRP.No significant effect was observed on the functional activity of the POMC neurons.