力学条件下Gli1+细胞参与上颌骨改建的作用研究

Gli1+cells contribute to bone formation during maxillary expansion

目的:观察神经胶质瘤关联癌基因同源物1阳性(Gli1+)细胞在Gli1-LacZ转基因小鼠上颌扩弓动物模型的组织分布情况,探讨其在上颌骨改建中的作用。方法:培育Gli1-LacZ转基因小鼠,构建小鼠上颌扩弓模型,应用HE及Masson染色观察上颌扩弓过程骨组织的改建,运用免疫荧光染色检测Gli1+细胞上颌骨中分布情况变化以及与Runt相关转录因子2(Runx2)之间的位置关系。结果:成功构建Gli1-LacZ转基因小鼠,将突变型用于后续实验。与对照组相比,上颌扩弓组腭中缝明显扩开,Gli1+细胞数量增加,Runx2表达增加,Gli1+细胞与Runx2共定位增加,成骨增强。结论:上颌分布的Gli1+细胞参与上颌扩弓力作用下的骨改建过程。

Objective:To verify the distribution of Gli1+cells in maxillary palatal suture,and to explore the role of Gli1+cells in osteogenesis induced by maxillary expansion,so as to provide experimental data to support the orthodontic treatment of skeletal malocclusion.Methods:Gli1-LacZ transgenic mice were established to detect the distribution of Gli1+cells in mid-palatal suture with or without maxillary expansion,HE and Masson staining were applied to evaluate the effect of maxillary expansion.In addition,immunofluorescence staining was used to observe the spatial relationship between Gli1+cells and the osteogenic marker Runx2 during maxillary expansion procedure.Results:Gli1-LacZ transgenic mice were successfully established and heterozygotes and mutants were used in the following experiments.Compared to the non-expansion group,the palatal suture of the maxillary expansion group was significantly widened,with increase of bone formation and the expression of osteogenic marker Runx2.Moreover,Gli1+cells increased in maxillary expansion group and colocalized with the Runx2 expression.Conclusions:Gli1+cells in maxillary palatal suture participate in osteogenesis induced by maxillary expansion.