基于TCGA和HADb数据库筛选自噬相关基因在舌癌中的差异表达及对预后的影响

Differential expression of autophagy related genes in tongue cancer and its influence on prognosis based on TCGA and HADb database

目的:探讨自噬相关基因(ATG)在舌癌发病机制中的作用,以建立一个基于ATG表达谱的舌癌患者预后预测模型。方法:基于TCGA和HADb数据库,采用Wilcox检验方法确定舌癌中差异表达的ATG,COX回归分析ATG与舌癌患者总生存率的相关性;GO、KEGG分析揭示舌癌中与自噬基因相关的信号通路和生物学过程;评价ATG与舌癌患者临床性状之间的关系。结果:舌癌组织和癌旁组织的ATG具有差异性表达,5个差异基因(VEGFA、ATG9B、WDR45、BAK1、VAMP3)与舌癌患者的总生存率显著相关,可预测患者预后生存;GO、KEGG分析显示多条关键自噬相关通路显著富集,如细胞凋亡、P53信号通路、自噬功能、表皮生长因子受体酪氨酸激酶抑制剂耐药等;ATG9B、VEGFA、WDR45等ATG与患者不同临床病理特征具有显著相关性。结论:VEGFA、ATG9B、WDR45、BAK1、VAMP3等差异自噬基因可预测舌癌的预后。

Objective:To explore the role of autophagy related genes(ATGs)in the pathogenesis of tongue cancer based on TCGA and HADb database,and to establish a prognosis prediction model of tongue cancer patients based on ATGs expression profile.Methods:The differential expression of ATGs in tongue cancer was determined by Wilcox test,and the correlation between ATGs and overall survival rate was analyzed by COX regression.Go and KEGG analysis revealed the signal pathway and biological process related to autophagy gene in tongue cancer.Finally,the relationship between ATGs and clinical characteristics of tongue cancer patients was evaluated.Results:ATGs in tongue cancer and adjacent tissues were differentially expressed.Five differentially expressed genes(VEGFA,ATG9B,WDR45,BAK1,VAMP3)were significantly related to the overall survival rate of patients with tongue cancer,which could predict the prognosis of survival.Go and KEGG showed significant enrichment of several key autophagy related pathways,such as apoptosis,P53 signaling pathway,autophagy function,EGFR tyrosine kinase inhibitor resistance,etc.Three ATGs(ATG9B,VEGFA,WDR45)were significantly correlated with different clinicopathological features.Conclusions:We identified five differential autophagy genes,VEGFA,ATG9B,WDR45,BAK1 and VAMP3,which can predict the prognosis of tongue cancer,and have reference value for autophagy research in the field of tongue cancer.