摘要
目的:研究慢性睡眠剥夺后大鼠颞下颌关节(TMJ)的组织学变化。方法:将60只大鼠随机分为对照组和实验组。采用改良多平台睡眠剥夺模型法对实验组大鼠进行4、6和8周的慢性睡眠剥夺,通过苏木精-伊红(HE)染色观察TMJ髁突组织病理学变化,CD105荧光染色观察血管新生情况,免疫组化染色检测TMJ髁突组织血管内皮生长因子(VEGF)的表达。结果:通过改良多平台法成功建立大鼠慢性睡眠剥夺模型;HE染色观察到实验组TMJ髁突组织出现纤维断裂,软骨细胞减少、排列紊乱,毛细血管数量增多等类骨关节炎(OA)样病理改变,而对照组无明显变化。实验组大鼠骨软骨交界处毛细血管密度(MVD)显著高于对照组(P<0.05),且表现出随睡眠剥夺时间延长而增加的趋势。免疫组化染色显示,VEGF在实验组的髁突软骨中层甚至浅层高度表达,而在对照组的髁突软骨各层中表达较低。结论:慢性睡眠剥夺后的大鼠可出现髁突软骨血管化、TMJ-OA样等病理改变,且随睡眠剥夺时间延长而逐渐加重。
Objective:To study the effects of chronic sleep deprivation on the histology of temporomandibular joint(TMJ)in rats.Methods:60 rats were randomly divided into control and experimental group.The rats were then chronicly deprived of sleep for 4,6 and 8 weeks by the modified multiple platform method(MMPM).Finally,the pathological changes,angiogenesis,and the expression of vascular endothelial growth factor(VEGF)of TMJ condylar cartilage were evaluated by hematoxylin and eosin(HE)staining,CD105 fluorescent staining,and immunohistochemistry,respectively.Results:MMPM can induce the rats into chronic sleep deprivation state successfully.HE staining showed osteoarthritic(OA)-like pathological changes,such as fiber fracture,reduced chondrocytes,disordered arrangement,and increased capillaries in the experimental group,while no changes were observed in the control group.The osteochondral junction micro vessel density of the experimental group were significantly higher than that of the control group(P<0.05),and showed increasing trend with the prolonged sleep deprivation.Immunohistochemisty showed that VEGF was highly expressed in the middle and even superficial layers of the condyle cartilage in the experimental group,while the expression was relatively lower in each layer of the condyle cartilage in the control group.Conclusions:Sleep deprivation of rats through MMPM could induce angiogenesis of TMJ condylar cartilage and TMJ-OA like histological changes in rats,which showed progressive enhancement in a time-dependent manner.
作者
王轲
赵兵杰
陈云
曹灵
WANG Ke;ZHAO Bingjie;CHEN Yun;CAO Ling(Jiangsu Key Laboratory of Oral Diseases,Nanjing 210029,China;Department of Endodontic,Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China;Nanjing Mingzhe Clinic,Nanjing 211500,China;Department of Pediatric and Preventive Dentistry,Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China)
出处
《口腔生物医学》
2020年第4期246-251,共6页
Oral Biomedicine
基金
南京医科大学科技发展基金(NMUB2018170)
江苏高校优势学科建设工程资助项目(2018-87)。
