右美托咪啶在南海近滩海水条件下对开放性颅脑损伤的抗炎和脑保护作用

Dexmedetomidine has anti-inflammatory and brain protective effects after open craniocerebral injury in the South China Sea near the beach

目的研究海战开放性脑损伤后使用右美托咪啶是否具有抗炎脑保护作用。方法选择新西兰兔12只,随机分为右美托咪啶组(Dex组)和生理盐水对照组(Con组),每组各6只。在地西泮麻醉下建立创伤性脑损伤模型。实验兔脑开放伤口经南海海水冲洗60 min后,Dex组实验兔腹腔注射50 mg/kg盐酸右美托咪啶,Con组给予相同剂量生理盐水。给药缝合好伤口后继续饲养48 h处死实验兔,取脑组织标本做HE染色。采外周血通过ELISA试剂盒检测白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)及中枢神经特异性蛋白(S100-β)指标水平变化。结果Con组创伤性脑细胞水肿主要表现为神经元和神经胶质细胞胞体明显肿胀,原有的椎体形状无法清晰辨认,细胞核浓染色,且出现细胞核周围环形低染甚至是空白染色区;而Dex组可明显改善以上组织细胞损伤和水肿的变化。ELISA法检测结果显示,和Con组相比,Dex组IL-1β、TNF-α和S100-β的水平明显降低,差异有统计学意义(P<0.05),而2组的IL-6水平变化比较差异无统计学意义(P>0.05)。结论颅脑开放性损伤后经南海近滩海水浸泡后给予右美托咪啶具有抗炎和脑保护作用。

Objective To investigate whether dexmedetomidine has anti-inflammatory brain protective effect after open brain injury in naval warfare.Methods Twelve New Zealand rabbits were selected and randomly divided into dexmedetomidine group(Dex group)and control group(Con group),with six rabbits in each group.The model of traumatic brain injury was established under diazepam anesthesia.The open wound of the experimental rabbit brain was washed with seawater in the South China Sea for 60 min.The rabbits in the Dex group were injected intraperitoneally with 50 mg/kg dexmedetomidine,and the Con group was given the same dose of normal saline.After administration of the sutured wound,the rabbits were sacrificed for 48 h.The brain tissue samples were taken for HE staining.Peripheral blood was collected to detect changes in interleukin 1β(IL-1β),interleukin 6(IL-6),tumor necrosis factorα(TNF-α),and S100-β indicators by ELISA kits.Results The traumatic brain cell edema in the Con group was mainly manifested by the obvious swelling of neurons and glial cell bodies,the original shape of the vertebral body could not be clearly identified,the nucleus was strongly stained,and there was a circular low staining or even a blank staining area around the nucleus;However,Dex group can significantly improve the above changes in all neurons damage and edema.The results of ELISA showed that compared with the Con group,the levels of IL-1β,TNF-α,and S100-β in the Dex group were significantly reduced,and the differences between the groups were statistically significant(P<0.05);While,there was no significant difference in IL-6 level between the two groups(P>0.05).Conclusion Anti-inflammatory and brain-protective effects of dexmedetomidine after seawater immersion in the South China Sea after open brain injury.