马齿苋中化学成分及其抗炎活性研究

The chemical constituents of Portulaca oleracea L.and their anti-inflammation activity

目的研究马齿苋中化学成分及其抗炎活性。方法采用脂多糖(LPS)诱导小鼠巨噬细胞RAW264.7建立的细胞炎症模型,以抗炎活性为导向结合大孔吸附树脂AB-8、反相硅胶ODS、Sephadex LH-20及半制备HPLC等色谱学方法对马齿苋乙醇提取物进行分离纯化,通过光谱学方法对所得化合物进行鉴定,对所得的化合物进行抗炎活性测试。结果分离鉴定了9个化合物。包括生物碱类化合物7个,分别为oleracein C(1)、D(2)、P(3)、Q(4)、H(5)、I(6)和E(7);其他类化合物2个,分别为(S)-5-carbethoxy-2-pyrrolidone(8)、(S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(9)。经抗炎活性评价发现,化合物1、3、5具有较好的抗炎活性。结论采用LPS诱导小鼠巨噬细胞RAW264.7建立的细胞炎症模型,可便捷地指导马齿苋抗炎活性物质的分离;首次利用该细胞模型从马齿苋乙醇提取物中筛选发现了3个具有较好抗炎活性的生物碱,为后续马齿苋抗炎活性成分的活体验证及深入开发利用提供了研究基础。

Objective To investigate the chemical constituents of purslane(Portulaca oleracea L.)and their anti-inflamma⁃tion activity.Methods The RAW 264.7 cells was used to create the lipopolysaccharide(LPS)-induced cellular inflammation assay model,which was used for the assay of anti-inflammatory activity in the present study.The separation of the ethanol extract of purslane and the isolation of compounds were performed by column chromatography over the macroporous adsorbent resin AB-8,reverse phase silica gel(ODS)and Sephadex LH-20,coupled with semi-preparative HPLC,under the guidance of the anti-inflammatory activity.The compounds obtained were identified by the spectroscopic data and tested for the anti-inflammation activity.Results Nine com⁃pounds were isolated from the ethanol extract of purslane,including seven alkaloids identified as oleracein C(1),D(2),P(3),Q(4),H(5),I(6)and E(7)as well as two other type compounds identified as(S)-5-carbethoxy-2-pyrrolidone(8)and(S)-5-hy⁃droxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(9).Among them,the compounds 1,3,and 5 showed a potent anti-inflammatory activ⁃ity in the cellular anti-inflammation assay.Conclusion The LPS-induced RAW 264.7 cellular inflammation assay model could be used to guide the isolation of anti-inflammatory substances from purslane,which has resulted in the isolation of three potent anti-in⁃flammatory compounds from purslane ethanol extract.The present research lays the foundation for further validation of in vivo activity and utilization of the anti-inflammatory compounds from purslane.