基于单克隆抗体的胶体金免疫层析法快速检测丙溴磷

Rapid Detection of Profenofos Using Colloidal Gold Immunoassay Based on Monoclonal Antibody

为实现蔬菜中丙溴磷的现场快速检测,采用免疫层析技术对丙溴磷残留的胶体金快速检测试纸条的制备开展研究。用胶体金颗粒标记丙溴磷单克隆抗体作为检测探针,以硝酸纤维素膜为固相载体,包被丙溴磷半抗原-卵清蛋白偶联物为检测线、羊抗鼠IgG为质控线,建立丙溴磷的胶体金快速检测试纸条。结果表明,该试纸条的检出限为0.10 mg/kg、灵敏度96%、特异性93%、假阴性率4%、假阳性率7%、相对准确度94.5%,重复性较好。胶体金试纸条操作简便、检测速度快、成本低,适用于蔬菜中丙溴磷残留的现场筛查和检测。

In order to realize the rapid detection of profenofos in vegetables,the preparation of colloidal gold strip for detection of profenofos was studied by immunochromatographic technique.Colloidal gold immunochromatographic strips for rapid detection of profenofos was established by using monoclonal antibody labeled with colloidal gold particles as detection probe,and nitrocellulose membrane was used as solid carrier,profenofos-OVA conjugate as the detection line and goat-anti-rat IgG as the control line.The results showed that the detection limit of the strip was 0.10 mg/kg.The sensitivity,specificity,false negative rate and false positive rate of the strip were 96%,93%,4%and 7%,respectively.The relative accuracy of the strip was 94.5%with good repeatability.The developed colloidal gold immunoassay is simple in operation,fast in detection,and low in cost,and is suitable for on-site screening and detection of profenofos residues in vegetables.