电感耦合等离子发射光谱法同时测定异甘草酸镁注射液中异甘草酸镁和氯化钠的含量

Establishment of inductively coupled plasma atomic emission spectrometry method for simultaneous determination of magnesium glycyrrhizinate and sodium chloride in the magnesium glycyrrhizinate injection

目的建立电感耦合等离子发射光谱(ICP-OES)法同时测定异甘草酸镁注射液中异甘草酸镁和氯化钠含量的新方法。方法2018年11月至2019年6月,采用ICP-OES法直接测定供试品稀释液,镁、钠的发射谱线分别为285.213、588.995 nm。结果镁离子、钠离子的线性范围分别为0.5~10.0μg/mL(r=0.9998)和15~100μg/mL(r=0.9992);镁离子、钠离子的平均加样回收率分别为96.73%、96.98%,RSD分别为1.87%和1.79%(n=6),3批样品ICP-OES法测定的结果与按标检验结果没有显著差异。结论该方法灵敏度高、专属性强、结果准确、操作简便,具有多元素同时测定的特点,可同时测定异甘草酸镁注射液中异甘草酸镁和氯化钠的含量,用于异甘草酸镁注射液的质量控制。

Objective To establish a new method for the simultaneous determination of the content of magnesium glycyrrhizinate and sodium chloride in the Magnesium glycyrrhizinate injection by inductively coupled plasma-atomic emission spectrometry(ICPOES).Methods From November 2018 to June 2019,ICP-OES was used to measure the diluent of the tested products,and the emission lines of Mg2+and Na+were 285.213 and 588.995 nm,respectively.Results The linear ranges of Mg2+and Na+were 0.5~10.0μg/mL(r=0.9998),15~100μg/mL(r=0.9992),respectively.The average recoveries of Mg2+and Na+were 96.73%,96.98%(n=6),and RSDs were 1.87%and 1.79%(n=6).The results of the three batches of sample showed that there were no significant difference between the ICP-OES method and the standard method.Conclusion This method has the characteristics of high sensitivity,strong speciality,high accuracy,simple operation,simultaneous determination of multiple elements,and can be used to determine the content of magnesium isoglycyrrhizinate and sodium chloride in Magnesium glycyrrhizinate injection.