微小核糖核酸-155、骨桥蛋白、嗜酸性粒细胞趋化因子水平与支气管哮喘病儿气管重塑的关系

Relationship between the levels of miRNA-155,OPN,eotaxin and airway remodeling in children with bronchial asthma

目的探讨微小核糖核酸-155(miRNA-155)、骨桥蛋白(OPN)、嗜酸性粒细胞趋化因子(Eotaxin)水平与支气管哮喘病儿气管重塑的关系及其联合检测对病情诊断的价值。方法选择成都市温江区人民医院于2016年5月至2018年12月期间收治的160例急性发作支气管哮喘病儿,根据高分辨率螺旋CT诊断结果是否发生气管重塑分为重塑组54例、非重塑组106例,纳入该院同期住院的其他呼吸系统疾病病儿60例为对照组。对入组病儿气管重塑指标、肺功能、miRNA-155、OPN及Eotaxin检测并对比。结果各组气管重塑指标和肺功能均有明显差异,其中对照组气管壁面积占气管总横断面积面积百分比(WA)(48.65±2.21)%,非重塑组WA(70.62±3.79)%,重塑组WA(86.44±5.02)%;重塑组miRNA-155低于非重塑组,对照组最高,均差异有统计学意义(P<0.05)。各组miRNA-155、OPN、Eotaxin指标均有明显差异,miRNA-155与WA呈负相关性,与一秒率(FEV1/FVC)、一秒用力呼气容积(FEV1)呈正相关;OPN、Eotaxin与WA呈正相关,与FEV1/FVC、FEV1呈负相关。结论联合检测miRNA-155、OPN、Eotaxin水平对支气管哮喘病儿气管重塑及病情严重程度评估具有一定的临床意义。

Objective To investigate the relationship between the levels of microRNA-155(miRNA-155),osteopontin(OPN)and eotaxin levels and tracheal remodeling in children with bronchial asthma and the value of combined detection in the diagnosis of the disease.Methods A total of 160 cases of acute bronchial asthma children,who were hospitalized in People’s Hospital of Chengdu Wenjiang District from May 2016 to December 2018,were selected and assigned into remodeling group(n=54)and nonremodeling group(n=106)according to the results of high resolution spiral CT in the diagnosis of whether there was airway remodeling.Meanwhile,60 cases of other respiratory inflammation admitted to hospital were included in control group.The tracheal remodeling indexes,lung function,miRNA-155,OPN and Eotaxin of the enrolled children were detected and compared.Results The trachea remodeling indexes and lung functions were significantly different among groups.The percentages of air wall area to total cross sectional area of trachea(WA)were(48.65±2.21)%in control group,(70.62±3.79)%in non-remodeling group and(86.44±5.02)%in remodeling group.MiRNA-155 in the remodeling group was lower than that in the non-remodeling group,with the highest in the control group,and there were statistically significant differences(P<0.05).MiRNA-155,OPN and Eotaxin indexes were significantly different among groups.MiRNA-155 was negatively correlated with WA and positively correlated with FEV1/FVC(one second rate)and FEV1(forced expiratory volume per second).OPN and Eotaxin were positively correlated with WA,while negatively correlated with FEV1/FVC and FEV1.Conclusion The combined detection of miRNA-155,OPN,and Eotaxin levels has certain clinical significance for airway remodeling in children with bronchial asthma and the evaluation of the severe condition of the disease.