响应面法优化牛蒡多糖超声辅助提取工艺与抗氧化活性评价

Optimization of Polysaccharides from Arctium Lappa L.by Ultrasound-Assisted Extraction Using Response Surface Methodology and Its Antioxidant Activities

以牛蒡多糖为研究对象,采用响应面法优化提取工艺,并进行了体外和细胞内抗氧化的活性评价。选取超声功率、超声时间、超声温度和料液比为考察指标,建立响应面法优化牛蒡多糖提取工艺,将获得的牛蒡多糖提取物进行结构表征、还原力、DPPH与ABTS+自由基清除能力、抗细胞氧化损伤等测试。结果表明,牛蒡多糖超声提取最优工艺为:超声时间20 min、料液比1:34(g/mL)、超声温度54℃、超声功率200 W,在此条件下,牛蒡多糖得率为(28.50±0.38)%。红外光谱表明牛蒡多糖为β-构型的呋喃糖。抗氧化活性结果显示,牛蒡多糖具有较强的抗氧化活性,在1000 mg/L时还原能力为0.130,对DPPH和ABTS+自由基清除率分别为55.93%和41.62%;在过氧化氢诱导的肝癌细胞Hep G2氧化损伤模型中,多糖在25~50μg/mL范围内有保护作用,50μg/mL组抗氧化效果最好。

The response surface method was used to optimize the extraction process of Arctium lappa L.polysaccharides and antioxidant activities were evaluated in vitro and intracellular.The effects of ultrasonic power,extraction time,extraction temperature,and solid to liquid ratio on extraction rate were scientifically investigated.Moreover,the obtained polysaccharide extracts was tested,including structure characterization,the reducing power,DPPH·and ABTS·scavenging ability,and the resistance to oxidative damage to cells.The best extraction condition were as follows:the extraction time of 20 min,the solid to liquid ratio of 1:34(g/mL),the extraction temperature of 54℃,and the ultrasonic power of 200 W.Under the optimal extraction condition,the extraction rate of polysaccharide was(28.50±0.38)%.What’s more,Fourier transform infrared spectrometry showed that furanose withβ-configuration glycosidic bonds was present in polysaccharide.In addition,the polysaccharides from Arctium lappa L.have strong antioxidative,the reducing power was 0.130,and the DPPH radical scavenging activities and the ABTS^+scavenging effect were 55.93%and 41.62%at 1000 mg/mL,respectively.On top of that,it has a protective effect on oxidative damage model of Hep G2 induced by hydrogen peroxide in the range of 25~50μg/mL and the best antioxidant concentration at 50μg/mL.