岩兰草挥发油抗氧化及酶抑制作用研究

Antioxidation and Enzyme Inhibition of Volatile Oil in Vetiver

使用水蒸气蒸馏法对岩兰草根部挥发油进行了提取,提取率为1.061%。气相色谱-质谱法(Gas Chromatography-Mass Spectrometry,GC-MS)分析结果显示,总计共鉴定得到43种挥发性化合物,占挥发油总量的98.26%,主要成分为(-)-异长叶烯(20.77%)、(-)-异雪松醇(10.79%)、β-杜松烯(5.80%)和β-石竹烯(5.73%)。抗氧化活性分析结果显示,该挥发油对1,1-二苯基-2-苦肼基、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐和超氧自由基的半数清除浓度(IC50)分别为(279.7±20.24)μg/mL、(20.8±3.03)μg/mL和(59.2±5.84)μg/mL。对脂肪酶和α-葡萄糖苷酶的抑制活性分析结果显示,该挥发油在质量浓度在150mg/mL时,对胰脂肪酶和α-葡萄糖苷酶活性抑制率分别为(99.56±0.28)%和(89.45±1.69)%,半数抑制浓度(IC50)分别为(45.9±5.84)mg/mL和(57.88±4.72)mg/mL。研究结果表明,岩兰草挥发油主要的化学成分为萜烯类、醇类等,具有较好的抗氧化能力,对胰脂肪酶和α-葡萄糖苷酶2种酶活性抑制效果显著。

The volatile oil from the roots of vetiver was extracted by steam distillation,and the extraction rate was 1.061%.Gas chromatography-mass spectrometry(GC-MS)analysis results showed that 43 kinds of volatile compounds had been identified,accounting for 98.26%of the total volatile oil,the main components were isolongifolene(20.77%),epi-cedrol(10.79%),β-cadinene(5.80%)and(-)-β-caryophyllene(5.73%).Antioxidant activity analysis results showed that the half-inhibitory concentration(IC50)of volatile oil of vetiver to 1,1-diphenyl-2-bitazide,2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium salt,and superoxide radical were(279.7±20.24)μg/mL,(20.8±3.03)μg/mL,and(59.2±5.84)μg/mL,respectively.The analysis results of the inhibitory activity of lipase andα-glucosidase showed that the volatile oil had a(99.56±0.28)%and(89.45±1.69)%inhibition rate of pancreatic lipase andα-glucosidase activity at a mass concentration of 150 mg/mL,the half-inhibitory concentration(IC50)were(45.9±5.84)mg/mL and(57.88±4.72)mg/mL,respectively.Experiments showed that the main chemical constituents in volatile oil of vetiver were terpenes and alcohols,which have good antioxidant capacity and have significant inhibitory effects on the enzyme activities of pancreatic lipase andα-glucosidase.