手术切除荷瘤淋巴结后小鼠肺组织内CD45^+、CD68^+、CD163^+和CD11b^+免疫细胞表达的变化

Changes of the expression of CD45^+,CD68^+,CD163^+and CD11b^+immune cells in mouse lung tissue after surgical resection of tumor-bearing lymph node

目的:探讨手术切除荷瘤淋巴结对小鼠远隔脏器肺组织内免疫细胞表达的影响。方法:将小鼠B16F10黑色素瘤细胞接种至小鼠髂下淋巴结(SiLN),15天后手术切除荷瘤淋巴结,所有小鼠分为手术切除SiLN组和未切除SiLN组(对照组)。应用HE及Elastic-Masson(EM)胶原纤维染色观察小鼠肺组织的一般形态结构和胶原纤维的变化;免疫组化染色观察肺组织中CD45^+、CD68^+、CD163^+和CD11b^+免疫细胞表达变化。结果:与对照组相比,手术切除SiLN组小鼠肺组织炎细胞浸润显著增加;血管周围胶原纤维稀疏;CD45^+总免疫细胞、CD68^+巨噬细胞、CD163^+M2型巨噬细胞和CD11b^+髓源性抑制性细胞(myeloid-derived suppressor cells,MDSCs)表达均显著增加(P<0.05)。结论:手术切除荷瘤淋巴结促进小鼠肺组织内CD163^+M2型巨噬细胞、CD11b^+MDSCs的表达增加,可能有利于远隔脏器肺组织内支持肿瘤细胞定植的炎性微环境的形成。

Objective:To investigate the effect of surgical resection of tumor-bearing lymph node on the expression of immune cells in mouse lung tissue.Methods:Mouse B16F10 melanoma cells were inoculated into mouse subiliac lymph node(SiLN).15 days later,tumor-bearing lymph node were surgically removed.All mice were divided into the surgical removal of SiLN group and the non-surgical removal group(control group).We used HE staining and Elastic-Masson staining to observe changes of general morphological structure and collagen fibers in mouse lung tissue,and applied immunohistochemistry to observe changes of CD45^+,CD68^+,CD163^+,CD11b^+immune cells in mouse lung tissue.Results:Compared with the control group,pulmonary inflammatory cells infiltration in the surgical removal of lymph node group was significantly increased.The stromal collagen fibers around the blood vessels in lung tissues were sparse.The expression of CD45^+total inflammatory cells,CD68^+macrophages,CD163^+M2 macrophages and CD11b^+myeloid-derived suppressor cells(MDSCs)in mouse lung tissue were significantly increased(P<0.05).Conclusion:Surgical removal of tumor-bearing lymph node can promote the expression of CD163^+M2 macrophages and CD11b^+MDSC in mouse lung tissue,which is helpful to the formation of distant organ microenvironment that supports tumor cell colonization.