胶质瘤组织中GRK5和Ki-67的表达情况及临床意义

Expression of GRK5 and Ki-67 in glioma tissues and analysis of its clinical significance

目的:探讨GRK5和Ki-67在胶质瘤组织中的表达情况,与胶质瘤临床病理特征之间的关系及GRK5和Ki-67在胶质瘤中的预后价值。方法:本研究收集2014年1月至2017年1月我院手术切除的104例脑胶质瘤患者的组织标本及同期因颅内损伤行颅内减压术手术切除的正常脑组织20例,采用免疫组化SP法检测胶质瘤组织及其正常脑组织中GRK5和Ki-67的表达水平。相关分析采用Spearman等级相关分析法。采用卡方检验分析GRK5和Ki-67在胶质瘤组织中的表达与临床病理特征的相关性。生存分析采用Kaplan-Meier和Log-rank检验,预后分析采用Cox比例风险模型。结果:通过免疫组化检测结果显示,GRK5在104例胶质瘤组织中阳性表达66例,阳性率为63.5%;在20例正常脑组织中阳性表达2例,阳性率为10.0%。Ki-67在104例胶质瘤组织中阳性表达69例,阳性率为66.3%;在20例正常脑组织中阳性表达率为0.0%。通过Spearman等级相关分析得出,胶质瘤组织中GRK5和Ki-67表达呈正相关(r=0.558,P=0.000)。分析GRK5和Ki-67的表达与胶质瘤患者临床病理特征之间的关系,结果显示GRK5和Ki-67的表达与WHO分级有关(P<0.05),与患者性别、年龄、肿瘤直径、肿瘤类型无关(P>0.05)。对104例患者进行生存分析,结果显示GRK5阳性表达患者生存率显著低于阴性表达患者(P=0.033);Ki-67阳性表达患者生存率显著低于阴性表达患者(P=0.033)。Cox回归模型分析结果显示,GRK5阳性表达和Ki-67阳性表达均是影响胶质瘤患者预后的独立危险因素(P<0.05)。结论:GRK5和Ki-67与胶质瘤的发生发展及细胞增殖有关,GRK5和Ki-67有可能作为胶质瘤患者的预后标志物及临床检测或联合检测的指标,提高临床诊断率。

Objective:To investigate the expression of GRK5 and Ki-67 in glioma tissues,the relationship between the expression of GRK5 and Ki-67 in gliomas,and the prognostic value of GRK5 and Ki-67 in gliomas.Methods:This study collected tissue samples from 104 glioma patients who were surgically resected from January 2014 to January 2017 in our hospital and 20 normal brain tissues that were surgically resected by intracranial decompression due to intracranial injury during the same period.Immunohistochemical SP method was used to detect the expression levels of GRK5 and Ki-67 in glioma tissue and normal brain tissue.Spearman rank correlation analysis was used for correlation analysis.The chi-square test was used to analyze the correlation between the expression of GRK5 and Ki-67 in glioma tissues and the clinicopathological characteristics.Kaplan-Meier and Log-rank tests were used for survival analysis,and Cox proportional risk model was used for prognostic analysis.Results:The results of immunohistochemical detection showed that 66 cases of GRK5 were positively expressed in 104 glioma tissues,with a positive rate of 63.5%.2 cases were positively expressed in 20 normal brain tissues,with a positive rate of 10.0%.Ki-67 was positively expressed in 104 glioma tissues in 69 cases,with a positive rate of 66.3%.The positive expression rate was 0.0%in 20 normal brain tissues.Spearman rank correlation analysis showed that GRK5 and Ki-67 expressions were positively correlated in glioma tissues(r=0.558,P=0.000).The relationship between the expression of GRK5 and Ki-67 and the clinicopathological characteristics of glioma patients was analyzed.The results showed that the expression of GRK5 and Ki-67 was related to WHO grading(P<0.05),but not to gender,age,tumor diameter and tumor type(P>0.05).Survival analysis of 104 patients showed that the survival rate of GRK5 positive expression was significantly lower than the negative expression(P=0.033).The survival rate of Ki-67 positive expression was significantly lower than the negative expression(P=0.033).Cox regression model analysis showed that both GRK5 positive expression and Ki-67 positive expression were independent risk factors affecting the prognosis of glioma patients(P<0.05).Conclusion:GRK5 and Ki-67 are related to the occurrence,development and proliferation of gliomas.GRK5 and Ki-67 may be used as prognostic markers and indicators of clinical detection or combined detection in glioma patients to improve the clinical diagnosis rate.