LncRNA PVT1在结直肠癌中的表达及其临床意义

The expression and its significance of LncRNA PVT1 in colorectal cancer

目的:探讨LncRNA PVT1在结直肠癌中的表达及其与临床病理特征之间的关系。方法:RT-qPCR法检测130例CRC患者癌组织和癌旁正常组织中LncRNA PVT1的表达,并揭示其表达的临床意义。结果:与癌旁正常组织相比较癌组织中的LncRNA PVT1呈高表达,其高表达与淋巴结转移和远处转移密切相关,而与T分级关系不大。LncRNA PVT1低表达和高表达患者的中位DFS分别为44个月和26个月(P=0.021);COX分析表明LncRNA PVT1表达水平和TNM分期均是预测DFS的独立影响因子。在Ⅰ期CRC中LncRNA PVT1高表达患者所占的比例显著高于血浆CEA升高患者的比例(P<0.0001)。结论:LncRNA PVT1在CRC的高表达与淋巴结转移和远处转移相关,是预测DFS的独立影响因子,另外其异常表达具有早期诊断CRC的潜在价值。

Objective:To investigate the association between LncRNA PVT1 expression and the clinicopathological characteristics in CRC patients.Methods:We detected the LncRNA PVT1 expression of cancerous tissues and paired-adjacent normal tissues in 130 patients with CRC by using RT-qPCR,and then revealed the clinical significance of LncRNA PVT1 expression in CRC.Results:The LncRNA PVT1 in CRC tissues had higher expression compared to paired-adjacent normal tissues and the overexpressed LncRNA PVT1 was closely related to lymph node metastasis and distant metastasis in CRC patients,but not to tumor T classification.The median DFS of the LncRNA PVT1 low and high expression groups was 44 months and 26 months,respectively(P=0.021).COX multivariate analysis showed that the LncRNA PVT1 expression and TNM stage were both independent prognostic predictors of DFS in patients with CRC.The proportion of patients with overexpressed LncRNA PVT1 was significantly higher than that of patients with elevated CEA in stage I of CRC patients(P<0.0001).Conclusion:The overexpressed LncRNA PVT1 is closely related to lymph node metastasis and distant metastasis which is an independent predictor of DFS in CRC patients.Additionally,the abnormal expression of LncRNA PVT1 has the potential value for early diagnosis of CRC.