受体相互作用蛋白激酶1在溃疡性结肠炎患者组织中的表达及意义

Expression and significance of receptor-interacting protein kinase 1 in ulcerative colitis patients

目的研究受体相互作用蛋白激酶1(RIPK1)在溃疡性结肠炎(UC)患者组织中的表达及与预后的关系。方法将纳入UC患者103例作为试验组,择同期行结直肠镜检查并切除肠息肉组织的58例患者作为对照组。用蛋白质印迹法检测UC组织中RIPK1水平,并分析其与UC严重程度及预后的关系。根据UC预后将其分为2组:预后不良亚组(n=39)和预后良好亚组(n=64)。用受试者工作特征(ROC)曲线评价RIPK1诊断UC预后的效能。用Logistic回归分析影响UC预后的相关因素。结果试验组RIPK1水平显著高于对照组(1.57±0.37 vs 0.82±0.16),差异有统计学意义(P<0.001)。预后不良组RIPK1水平高于预后良好组(1.90±0.32 vs 1.38±0.23),差异有统计学意义(P<0.001)。RIPK1评价UC活动期严重程度(中、重度)的ROC曲线下面积(AUC)为0.93(95%CI:0.86~0.97),最佳截断点、敏感度和特异度为分别1.41,92.42%和83.78%;RIPK1评价UC预后的ROC AUC为0.91(95%CI:0.84~0.96),最佳截断点、敏感度和特异度分别为1.59,84.62%和84.37%;RIPK1是UC预后的独立影响因素(P<0.05)。结论RIPK1在UC组织中高表达,是影响UC病情及预后不良的影响因素。

Objective To investigate the expression of receptor-interacting protein kinase 1(RIPK1)in ulcerative colitis(UC)patients tissues and its relationship with prognosis.Methods A total of 103 UC patients were selected as treatment group.A total of 58 patients who underwent colorectal examination and intestinal polypectomy were selected as control group.Western blot was used to detect the level of RIPK1 in UC tissues,and its relationship with the severity and prognosis of UC was analyzed.According to the prognosis of UC,they were divided into two groups:Poor prognosis subgroup(n=39)and good prognosis subgroup(n=64).Receiver operating characteristic(ROC)curve was used to evaluate the efficacy of RIPK1 in the diagnosis of UC.Logistic regression analysis was used to analyze the related factors affecting the prognosis of UC.Results The level of RIPK1 in treatment group was higher than control group(1.57±0.37 vs 0.82±0.16),the difference was significant(P<0.001).The level of RIPK1 in poor prognosis group was higher than good prognosis group(1.90±0.32 vs 1.38±0.23)the difference was significant(P<0.001).The ROC area under curve(AUC)of RIPK1 to evaluate the severity and prognosis of UC active period(moderate and severe)was 0.93(95%CI from 0.86 to 0.97),the best cutoff point,sensitivity and specificity were 1.41%,92.42%and 83.78%,respectively.The ROC AUC of RIPK1 to evaluate the severity and prognosis of prognosis of UC was 0.91(95%CI:0.84 to 0.96),the best cutoff point,sensitivity and specificity were 1.59,84.62%and 84.37%,respectively.RIPK1 was an independent risk factor for UC prognosis(all P<0.05).Conclusion The expression of RIPK1 was up-regulated in UC tissues and severed as a factor in severity and poor prognosis of UC.