扶正祛毒方对慢性乙型肝炎病毒携带小鼠细胞免疫水平的调节作用

Regulating effect of Fuzheng Qudu Decoction on cellular immunity of mice carrying chronic hepatitis B virus

目的观察扶正祛毒方对慢性乙型肝炎病毒(HBV)携带小鼠细胞免疫水平的调节。方法选择雄性HBV转基因C57BL/6J小鼠30只,按随机数字表法将其随机分为模型组、西药组、中药组,每组各10只,同系雄性正常(非转基因)小鼠10只作为空白组。中药组予扶正祛毒方药液0.10 mL/(10 g·d)灌胃,西药组予阿德福韦酯药液0.08 mL/(10 g·d)灌胃,模型组及空白组予生理盐水0.10 mL/(10 g·d)灌胃,均为1次/d。24周后处死取材,用流式细胞仪检测小鼠外周血中CD8+T淋巴细胞、CD4+T淋巴细胞所占比例,并计算CD4^+T/CD8^+T;用酶联免疫吸附法测定肝组织中白细胞介素(IL)-2、IL-4水平,间接反映辅助性T细胞(Th)中的Th1与Th2比例。结果中药组CD8^+T淋巴细胞比例高于模型组(P<0.05);模型组CD4^+T/CD8^+T高于空白组(P<0.05);西药组和中药组CD4^+T/CD8^+T低于模型组(P<0.05);模型组IL-2水平高于空白组(P<0.05),中药组IL-2水平高于模型组和西药组(P<0.05);模型组IL-4水平高于空白组(P<0.05);西药组和中药组IL-4水平低于模型组(P<0.05)。结论扶正祛毒方能提高慢性HBV携带小鼠的细胞免疫水平,其作用机制可能与调节Th1/Th2比例有关。

Objective To observe the regulation of Fuzheng Qudu Decoction on cellular immunity of mice carrying chronic hepatitis B virus(HBV).Methods Thirty male HBV transgenic C57BL/6J mice were selected,and they were randomly divided into the model group,the Western medicine group,and the traditional Chinese medicine group according to the random number table method,with 10 mice in each group,and 10 normal male(non-transgenic)mice of the same line as the blank group.The traditional Chinese medicine group was given Fuzheng Qudu Decoction 0.10 mL/(10 g·d)by gavage,the Western medicine group was given Adefovir Dipivoxil 0.08 mL/(10 g·d)by gavage,the model group and the blank group were given physiological saline 0.10 mL/(10 g·d)by gavage,and all groups were administered once every day.After 24 weeks,the mice were sacrificed and taken,and the proportions of CD8+T lymphocytes and CD4^+T lymphocytes in the peripheral blood of the mice were detected by flow cytometry,and the CD4^+T/CD8^+T was calculated.Enzyme-linked immunosorbent assay was used to measure the levels of interleukin(IL)-2 and IL-4 in liver tissue,which indirectly reflected the ratio of Th1 to Th2 in helper T cells(Th).Results The proportion of CD8+T lymphocytes in the traditional Chinese medicine group was higher than that in the model group(P<0.05).CD4^+T/CD8^+T in the model group was higher than that in the blank group(P<0.05).CD4^+T/CD8^+T in the Western medicine group and the traditional Chinese medicine group were lower than that in the model group(P<0.05).The level of IL-2 in the model group was higher than that in the blank group(P<0.05),and the level of IL-2 in the traditional Chinese medicine group was higher than that in the Western medicine group and the model group(P<0.05).The level of IL-4 in the model group was higher than that in the blank group(P<0.05),and the level of IL-4 in the the Western medicine group and the traditional Chinese medicine group were lower than that in the model group(P<0.05).Conclusion Fuzheng Qudu Decoction can improve the cellular immune level of chronic HBV carrying mice,its mechanism may be related to the regulation of the proportion of Th1/Th2.