生物炭水凝胶固定微生物处理含酚废水

Study on the treatment of phenolic wastewater by carbonaceous hydrogels immobilized microorganisms

筛选和分离出一种高效的苯酚降解菌Bacillus sp.26,以西瓜生物炭水凝胶作为载体,用海藻酸钠作为交联剂包埋该菌株.通过实验研究对比灭活的固定化Bacillus sp.26细胞对苯酚的吸附;固定化Bacillus sp.26细胞对苯酚的吸附和降解;游离Bacillus sp.26细胞对苯酚的降解能力.结果表明:Bacillus sp.26游离菌株具有高效降酚能力,可在1.0 d内降解浓度高达800 mg/L的高盐含酚废水;生物炭水凝胶固定化Bacillus sp.26同样显示出高效降解苯酚废水的能力,且具有优良的循环使用性能,在循环使用3次后,对1200 mg/L苯酚废水的降解效率仍达到100%;固定化后的Bacillus sp.26在循环使用过程中性能不断提高,说明炭水凝胶为菌株提供良好的生长和增殖环境.生物炭水凝胶固定微生物的方法具有易于回收、分离和重复使用的特点,具有潜在的应用价值.所筛选出的菌株和发展的固定化方法,为固定化微生物技术处理苯酚废水提供新的材料和思路.

A highly efficient phenol-degrading bacterium Bacillus sp.26 was screened and isolated.The watermelon derived biochar hydrogel was used as a carrier,and calcium alginate was used as a cross-linking agent to embed the strain.Experiments were conducted to compare the adsorption of phenol by inactivated and immobilized Bacillus sp.26 cell.Adsorption and degradation of phenol by immobilized Bacillus sp.26 cells and degradation of phenol by free Bacillus sp.26 cells were studied.The results show that the free strain of Bacillus sp.26 has efficiency for the degradation of high-salt phenol-containing wastewater(800 mg/L within 1.0 day);biochar hydrogel immobilized Bacillus sp.26 also shows the ability to efficiently degrade phenol wastewater with excellent recycling performance.In the third cycle,the efficiency of degrading 1200 mg/L phenol still reached 100%.The immobilized Bacillus sp.26 showed an enhanced performance during the recycling process,indicating that the biochar hydrogel provides an appropriate environment for growth and proliferation of the strains.Immobilization of microbial by biochar hydrogel has the characteristics of easy recovery,separation and reuse,which endows it with great application potential.The new strains and the developed microbial immobilization method provide new materials and technologies for the treatment of phenol wastewater.