不明原因复发性流产患者血清细胞因子的变化

Changes in serum cytokine profiles of patients with unexplained recurrent spontaneous abortion

目的:观察不明原因复发性流产者血清趋化因子1(I-309)、巨噬细胞炎性蛋白1α(MIP-1α)、趋化因子15(MIP-1δ)、干扰素-γ诱导的单核因子(MIG)、白细胞介素15(IL-15)、趋化因子11(CCL11)、肿瘤坏死因子可溶性受体2(sTNFR2)的变化,探讨不明原因复发性流产的发病机制。方法:采用Aimplex流式高通量多因子检测技术分别检测不明原因复发性患者和健康生育者I-309、MIP-1α、MIP-1δ、MIG、IL-15、CCL11、sTNFR2的血清水平。结果:不明原因复发性流产患者与健康生育者血清I-309分别为[6.0(4.1,12.5)、2.9(2.5,4.9)]pg/ml,MIP-1δ分别为[1625.3(1297.2,1897.7)、981.7(912.3,1151.1)]pg/ml,MIG分别为(5.2±3.5)、(2.1±0.9)pg/ml,sTNFR2分别为[(306.9(228.4,377.6)、162.0(143.9,204.2)]pg/ml,不明原因复发流产患者均明显升高(P<0.001);MIP-1α分别为[6.9(4.6,11.6)、22.7(12.4,38.4)]pg/ml,不明原因复发流产患者明显降低(P<0.001)。结论:I-309、MIP-1α、MIP-1δ、MIG、sTNFR2异常可能与不明原因复发性流产的发病机制有关。

Objective:To observe the changes of serum cytokine,such as Serum I-309,macrophage inflammatory protein 1α(MIP-1α),MIP-1δ,MIG,interleukin-15(IL-15),CCL11,and tumor necrosis factor soluble receptor 2(sTNFR2)of patients with unexplained recurrent spontaneous abortion(RSA),and to discuss the pathogenesis of unexplained RSA.Methods:Aimplex bead-based immunoassays were applied to detected the serum concentration of I-309,MIP-1α,MIP-1δ,MIG,IL-15,CCL11,and sTNFR2 of patients with RSA and healthy fertile women.Results:The concentrations of I-309,MIP-1δ,MIP-1δ,MIG,and sTNFR2 of patients with RSA were 6.0(4.1,12.5)pg/ml,1625.3(1297.2,1897.7)pg/ml,5.2±3.5 pg/ml,and 306.9(228.4,377.6)pg/ml,respectively,which were significant higher than those 2.9(2.5,4.9)pg/ml,981.7(912.3,1151.1)pg/ml,2.1±0.9pg/ml,and 162.0(143.9,204.2)pg/ml,respectively)of healthy fertile women,but the concentration of MIP-1αof patients with RSA was 6.9(4.6,11.6)pg/ml,which was significant higher than that[22.7(12.4,38.4)pg/ml]of healthy fertile women(all P<0.001).Conclusion:The abnormalities of I-309,MIP-1α,MIP-1δ,MIG,and sTNFR2 levels of patients with RSA may be related to the pathogenesis of RSA.