VEGFb参与白藜芦醇促进面神经损伤大鼠神经再生

VEGFb involves in resveratrol promoting nerve regeneration in rats with facial nerve injury

目的探索白藜芦醇(Res)在面神经损伤模型中的作用及机制。方法按照随机分组原则将雄性SD大鼠分为假手术组(Sham组)和面神经损伤组(Crush组),对Crush组大鼠行右侧面神经干夹持损伤建立面神经损伤模型。将Crush组大鼠随机分为3组:对照组(Crush+Vehicle组)、白藜芦醇组(Crush+Res组)以及抑制剂组(Crush+Res+Ax-itinib组)。采用Westernblot和QPCR比较不同组大鼠面神经内血管内皮生长因子b(VEGFb)及其受体VEGFR1、VEG-FR2的表达。采用免疫组化对面神经损伤及再生情况进行评估。结果与Crush+Vehicle组相比,Crush+Res组VEGFb、VEGFR1、VEGFR2的表达提高(n=3,P<0.05),神经再生得到促进。与Crush+Res组相比,Crush+Res+Ax-itinib组中VEGFb及VEGFR1、VEGFR2的表达和神经再生作用被抑制(n=3,P<0.01)。结论Res在面神经损伤后提高了VEGFb、VEGFR1和VEGFR2的表达,促进神经再生。

Objective To explore the role and mechanism of resveratrol(Res) in facial nerve injury model. Methods Male SD rats were randomly divided into sham operation group(Sham group) and facial nerve injury group(Crush group), and facial nerve injury model was established by clamping injury of right facial nerve trunk in rats of the Crush group. The rats in the Crush group were randomly divided into three groups: the control group(Crush+Vehicle group), the resveratrol group(Crush+Res group), and the inhibitor group(Crush+Res+Axitinib group). Western blot and QPCR were used to compare the expression levels of vascular endothelial growth factor b(VEGFb) and its receptors 1 and 2(VEGFR1, VEGFR2) in facial nerve of different groups. Immunohistochemistry was used to evaluate facial nerve regeneration. Results Compared with the Crush+Vehicle group, the expression of VEGFb, VEGFR1 and VEGFR2 in the Crush+Res group was increased and nerve regeneration was promoted. The expression of VEGFb, VEGFR1 and VEGFR2 in Crush+Res+Axitinib group was inhibited compared with that in Crush+Res group. Conclusion Res can enhance the expression of VEGFb, VEGFR1 and VEGFR2 after facial nerve injury and promote nerve regeneration.