CCL7过表达促进肺癌细胞A549的增殖和侵袭

Overexpression of CCL7 promoted proliferation and invasion of A549 cell line in lung cancer

目的:通过上调CCL7的表达,观察其对肺癌细胞A549增殖、迁移及侵袭等生物学功能的影响,探讨CCL7在肺癌转移中发挥的作用。方法:慢病毒包装CCL7过表达载体感染A549细胞系,分别进行MTT、划痕实验和Transwell小室细胞侵袭实验。结果:MTT实验显示CCL7过表达促进了A549细胞增殖(P<0.01)。划痕实验表明CCL7过表达可以促进A549细胞迁移(P<0.01)。同时Transwell小室侵袭实验证实过表达CCL7的A549细胞具有更强的侵袭能力(P<0.05)。结论:CCL7的过表达可以促进肺癌细胞系A549的增殖、迁移和侵袭,对CCL7的进一步研究可以为肺癌基础研究及诊断治疗提供新的方向。

Objective:To investigate the biological functions of A549 cell line by up-regulating CCL7 and explore the role of CCL7 in lung cancer metastasis.Methods:A549 cell line was infected by CCL7 overexpressing lentiviral vector.MTT,scratch test and Transwell cell invasion assay were performed.Results:MTT assay showed that CCL7 overexpression promoted proliferation of A549 cells(P<0.01).Scratch experiments showed that CCL7 can promote the migration of A549 cells(P<0.01).At the same time,Transwell chamber invasion experiments confirmed that A549 cells with overexpression of CCL7 had stronger invasive ability(P<0.05).Conclusion:The high expression of CCL7 can promote the proliferation,migration and invasion of lung cancer cells.Further research on CCL7 may provide new directions for basic research,diagnosis and treatment of lung cancer.