miR-200a在子宫内膜腺癌患者组织及血清中的表达及临床意义

The expression and clinical significance of the microRNA-200a in the tissue and serum samples from the patients with endometrial adenocarcinoma

目的:研究miR-200a在子宫内膜腺癌中的表达变化及其与肿瘤分期之间的关系,为子宫内膜腺癌的早期临床检测及诊断提供新的思路。方法:本实验以正常子宫内膜、子宫肌瘤患者子宫内膜和子宫内膜腺癌的组织和患者血清标本为研究对象。通过PCR方法分别检测了各组患者血清及子宫内膜组织中miR-200a的表达,并分析了miR-200a在子宫内膜腺癌中的表达变化及其与肿瘤分期之间的关系。结果:miR-200a在子宫内膜腺癌标本中的表达明显高于正常的子宫内膜组织,平均升高约9.1倍(P<0.05),而在血清标本中平均升高约5.4倍(P<0.05)。Ⅲ、Ⅳ期子宫内膜腺癌患者血清标本中miR-200a的含量为7.7±1.9,显著高于早期(Ⅰ、Ⅱ期)患者中的4.2±1.9(P<0.05)。结论:miR-200a在子宫内膜腺癌患者的组织标本及血清中表达均显著高于正常,可以作为子宫内膜腺癌的一个重要的肿瘤标记物,为子宫内膜腺癌的筛查及检测提供了一种有效方法。

Objective:To explore the difference of microRNA-200a expression in endometrial adenocarcinoma and its relationship with the staging of the tumor,so as to provide a new method for early detection and diagnosis of endometrial adenocarcinoma.Methods:The tissue samples from normal endometrium and endometrial adenocarcinoma patients as well as their serum sample were studied.The expression of microRNA-200a in serum and endometrial tissues was detected by PCR,and the relationship between the expression of microRNA-200a and the tumor stage of endometrial adenocarcinoma was analyzed.Results:The expression of microRNA-200a in endometrial adenocarcinoma specimens was significantly higher than that in normal endometrial tissues,with an average increase of 9.1 times(P<0.05)in tissue specimens and 5.4 times(P<0.05)in serum specimens.The serum level of microRNA-200a in patients with stage III and IV endometrial adenocarcinoma was 7.7±1.9,which was significantly higher than that in early stage(stage I and II)patients(4.2±1.9)(P<0.05).Conclusion:The expression of microRNA-200a in tissue and serum from endometrial adenocarcinoma patients is significantly higher than that of normal.It might be used as an important tumor marker for endometrial adenocarcinoma,and provide an effective method for early screening and detection of endometrial adenocarcinoma.