温度、盐度和光照强度对坛紫菜壳孢子放散的影响

Effects of temperature,salinity and light intensity on conchospore releasing of Pyropia haitanensis

坛紫菜(Pyropia haitanensis)是中国暖温带海区特有的一种规模化人工栽培的经济红藻。坛紫菜壳孢子采苗是人工栽培的关键环节,壳孢子放散量对栽培生产的产量形成至关重要。本文采用正交试验设计[L25(53)],研究了温度、盐度和光照强度对坛紫菜成熟贝壳丝状体放散壳孢子的影响。结果表明,温度对壳孢子放散量有显著影响(F=8.584,P=0.002<0.05);盐度、光照强度对壳孢子放散量影响不显著(F=1.073,P=0.412>0.05;F=0.585,P=0.680>0.05)。方差分析显示,温度对坛紫菜壳孢子放散量的影响极显著(F=9.215,P<0.01)。温度是一阶主效应,与盐度、光照强度二阶交互效应不显著(P>0.05)。坛紫菜壳孢子放散的最优环境条件为温度26℃、盐度1.018~1.023、光照强度5000~8000 Lux。

Pyropia haitanensis is an economically important cultivated seaweed in China.Conchospore releasing is a critical event of artificial cultivation of Pyropia,consequently the amount of conchospore released is very important to the success of the seedling collection.Based on an orthogonal design with 3 factors and 5 levels,the effects of temperature,salinity and light intensity on the conchospore releasing of P.haitanensis were studied.The results showed that temperature had a significant effect on the amount of conchospore released(F=8.584,P=0.002<0.05);salinity and light intensity had no significant effects on the amount of conchospores(F=1.073,P=0.412>0.05;F=0.585,P=0.680>0.05).Analysis of variance showed that temperature had an extremely significant effect on the amount of conchospore released from P.haitanensis(F=9.215,P<0.01).Temperature was the first-order main effect,and the second-order interaction effect with salinity and light intensity was not significant(P>0.05).The optimal environmental conditions for the release of conchospore were as follows:temperature of 26℃,salinity of 1.018~1.023,and light intensity of 5000~8000 Lux.