石房蛤毒素胶体金快速检测试纸卡的应用研究

Study on the application of colloidal gold rapid test strip for detection of saxitoxin

麻痹性贝类毒素是世界范围内分布最广、危害最大的一类赤潮生物毒素,其主要毒素为石房蛤毒素。本研究对一种石房蛤毒素胶体金快速检测试纸卡进行了实际应用测试。经试验,该试纸卡在贝类产品中具有普遍适用性,对石房蛤毒素的检测限为500μg/kg。同时,用试纸卡对30个不同贝类实际样品中的石房蛤毒素进行检测,结果与液相色谱-串联质谱法完全一致,单个样品检测耗时约20 min。结果表明,该试纸卡具有较高的灵敏度和特异性,操作便捷、稳定可靠,能够满足现场检测需要,可供基层监管部门开展麻痹性贝毒快速检测筛查工作,对提高赤潮期间贝类质量安全风险防控能力具有重要意义。

Paralytic shellfish poisoning is one of the most widely distributed and harmful biological toxins of red tide in the world,and the main toxin is saxitoxin.In this study,a kind of colloidal gold rapid test strip for detection of saxitoxin was tested in practice.According to the test,the test strip could be universally applied in shellfish,and the detection limit of saxitoxin was 500μg/kg.Meanwhile,the test results of saxitoxin in 30 samples of different shellfish were completely consistent with that of liquid chromatography-tandem mass spectrometry.It took about 20 minutes to test a single sample.The results showed that the test strip had high sensitivity and specificity,convenient operation,stability and reliability,which could meet the needs of on-site detection.It was available for the grass-roots fishery regulatory department to carry out the rapid detection and screening of paralytic shellfish poisoning,which was of great significance for strengthening the ability to prevent and control the quality and safety risks of shellfish during the red tide.