HPLC对多叶棘豆中7个苷类化合物的定量分析

Quantitative Analysis of Seven Glycosides in Oxytropis Myriophylla by HPLC

目的建立多叶棘豆中7个苷类化合物的定量分析方法。方法以山奈酚-3-O-β-D-葡萄糖苷、异鼠李素-3-O-β-D-葡萄糖苷、(3R,4R)-4,7-二羟基-2’,3’-二甲氧基黄烷-4’-O-β-D-葡萄糖苷、3-羟基苯甲醇-4-O-β-D-葡萄糖苷、6-甲氧基香豆素-7-O-β-D-葡萄糖苷、2-羟基-6-甲氧基苯乙酮-4-O-β-D-葡萄糖苷和苯乙酮-4-O-β-D-葡萄糖苷为评价指标,采用HPLC对不同产地多叶棘豆进行定量分析。色谱柱:Topsil C18(250 mm×4.6 mm,5μm);流动相:乙腈-水,梯度洗脱;检测波长:261 nm;流速:1.0 mL·min–1;柱温为30℃;进样量为20μL。结果7个苷类化合物在一定范围内均具有较好的线性关系(r>0.9992),其平均回收率分别为96.0%,97.0%,98.7%,96.0%,96.0%,97.7%,98.0%,RSD均<2.0%。被检测成分的含量变化分别为0.192~0.310,0.215~0.325,0.504~0.753,0.128~0.398,0.151~0.217,0.354~0.550,0.132~0.385 mg·g–1。结论本实验所建立的含量测定方法可为综合评价该药材质量提供参考。

OBJECTIVE To establish the quantitative analysis method for seven glycosides in Oxytropis myriophylla.METHODS Kaempferol-3-O-β-D-glucoside,isorhamnetin-3-O-β-D-glucoside,(3R,4R)-4,7-hydroxy-2’,3’-dimethoxyisoflavane-4’-O-β-D-glucoside,3-hydroxybenzyl alcohol-4-O-β-D-glucoside,6-methoxycoumarin-7-O-β-D-glucoside,2-hydroxy-6-methoxyacetophenone-4-O-β-D-glucoside and acetophenone-4-O-β-D-glucoside was taken as evaluation indexes,the quantitative analysis of Oxytropis myriophylla from different habitats was determined by HPLC.The chromatographic column:Topsil C18(250 mm×4.6 mm,5μm).The mobile phase was comprised of acetonitrile and water with a gradient elution.The detection wavelength was 261 nm with flow rate at 1.0 mL·min–1,column temperature at 30℃and injection volume at 20μL.RESULTS The 7 glycosides all had a good linearity within a certain range(r>0.9992).The average recoveries were 96.0%,97.0%,98.7%,96.0%,96.0%,97.7%,98.0%,respectively,all of RSDs were<2.0%.Tested compounds and their content change in different samples were 0.192−0.310,0.215−0.325,0.504−0.753,0.128−0.398,0.151−0.217,0.354−0.550,0.132−0.385 mg⋅g–1,respectively.CONCLUSION The content detection method established in this experiment can provide reference for comprehensive evaluation of the quality of this medicinal material.