北败酱草镇痛、抗炎及体外抑菌作用探讨

Analgesic,Anti-inflammatory and Antibacterial Effects in Vitro of Beibaijiangcao

目的:探讨北败酱草在抗炎镇痛方面的药理作用及可能的机制。方法:以醋酸致小鼠扭体法、热板法探索北败酱镇痛作用,以二甲苯致小鼠耳肿胀、体外抑菌实验探讨其抗炎作用。结果:与给药前比较,小鼠舔足跳跃反应潜伏时间阿司匹林组、北败酱草高、中剂量组分别在给药后1、2、3 h增加,差异有统计学意义(P<0.05);与模型对照组相同时间点比较,小鼠舔足跳跃反应潜伏时间阿司匹林组给药后2、3 h和北败酱草提取物高、中剂量组给药后1、2、3 h均增加,与阿司匹林组比较,北败酱草低剂量组给药1、2、3h均增加,差异有统计学意义(P<0.05);与模型对照组比较,阿司匹林组及北败酱草高、中、低剂量组小鼠扭体次数均降低,与阿司匹林组比较,北败酱草中、低剂量组扭体次数降低,差异有统计学意义(P<0.05);与模型组对照组比较,阿司匹林组及北败酱草提取物高、中剂量组小鼠耳廓肿胀度降低,与阿司匹林组比较,北败酱草低剂量组耳廓肿胀度升高,差异均有统计学意义(P<0.01);北败酱草对痢疾杆菌、大肠杆菌、金黄色葡萄球菌的最小抑菌浓度分别为1.25、1.25、0.1562g生药量/mL。结论:北败酱草提取物有抗炎、镇痛和体外抑菌作用。

Objective: To discuss the potential mechanism and pharmacological effects of Beibaijiangcao on anti-inflammation and analgesis. Methods: Analgesic effects of Beibaijiang were explored through acetic acid induced writhing test and hot plate test in mice, and its anti-inflammatory effects discussed through xylene-induced mouse ear swelling and external bacteriostatic experiment. Results: Compared with before medication, the latency of licking foot jump reaction increased in aspirin group, high and maderate dose groups of Beibaijiangcao one, two and three hours after medication, compared with aspirin group, it rose in low dose group of Beibaijiangcao one, two and three hours after medication, and the difference showed statistical meaning(P<0.05);compared with model control group, the writhing times reduced in aspirin groups, high, moderate and low dose groups of Beibaijiangcao,compared with aspirin group, the writhing times lowered in moderate and low dose groups of Beibaijiangcao, the difference showed statistical meaning(P <0.05);compared with model control group, auricle swelling degree decreased in aspiring group, high and moderate dose groups, compared with aspirin group, the degree lifted in low dose group, the difference had statistical meaning(P<0.01), the minimum inhibitory concentrations to dysentery bacilli, Escherichia coli and staphylococcus aureus were 1.25, 1.25 and 0.1562 g crude drug/mL. Conclusion: The extract of Beibaijiangcao owns anti-inflammation, analgesis and external bacteriostatic effects.