玫瑰花颗粒对抑郁小鼠行为学及血清5-HT的影响

Effects of Rose Granules on behavior and serum 5-HT in depressed mice

观察玫瑰花颗粒抗抑郁行为的作用,并初步探讨其治疗抑郁症可能的作用机制.选用健康、雄性成年ICR小鼠60只,采用21 d孤养结合慢性不可预见性温和刺激方法造模;分别在给药7 d、14 d、28 d通过体重变化、悬尾实验(TST)、强迫游泳实验(FST)和旷场实验(OFT)观察小鼠抑郁行为,采用Elisa法检测血清5-羟色胺(5-HT)的变化.结果发现,用药前后各组小鼠体重均无明显变化;玫瑰花颗粒高、中剂量组能明显缩短小鼠强迫游泳不动时间;玫瑰花颗粒高剂量组能显著减少小鼠悬尾不动时间;玫瑰花颗粒各剂量组均能明显增加小鼠旷场实验的总运动距离;给药7 d,玫瑰花颗粒各剂量组均能明显增加小鼠进入中心方格的次数;且玫瑰花各剂量组均能显著升高小鼠血清5-HT含量.玫瑰花颗粒可以改善抑郁模型小鼠的行为,且能显著提高小鼠血清中5-HT的含量,提示玫瑰花颗粒可能是通过影响5-HT水平改善抑郁.

To observe the antidepressant effect of Rose Granules,and preliminarily explore the possible mechanism of its treatment of depression.In this study,60 healthy,male adult ICR mice were selected.The 21 days orphan rearing combined with chronic unpredictable mild stimulation was used to create the model;The depressive behavior of mice was observed by weight change,tail suspension test(TST),forced swimming test(FST)and open field test(OFT)on 7 d,14 d and 28 d,respectively,and the serum serotonin(5-hydroxytryptamine,5-HT)change.The results showed that there was no significant change in the body weight of mice in each group before and after the medication;the high and medium doses of the rose flower granule group can significantly shorten the time of forced swimming immobility;the high dose of rose flower granule group can significantly reduce the tail suspension of mice Time;each dosage group of rose flower granules can significantly increase the total movement distance of mice in the open field experiment;7 days of administration,each dosage group of rose flower granules can significantly increase the number of times mice enter the center grid;and each dose of rose flower Both groups can significantly increase the serum 5-HT content of mice.Rose Granules can improve the behavior of depression model mice,and can significantly increase the 5-HT content in the serum of mice,suggesting that Rose Granules may improve depression by affecting the 5-HT level.