龙须菜中硼的化学形态分析

Speciation analysis of boron in Gracilariopsis lemaneiformis

为探究硼对大型海藻龙须菜食用安全性的影响,本文以福建沿海龙须菜为原料,采用超声辅助连续浸提和电感耦合等离子发射谱(ICP-OES)相结合的方法,探究龙须菜中硼的分布及存在形态,并对浸提条件进行优化.优化浸提参数如下:将龙须菜粉碎至20~30目,采用0.2 mol·L^-1NaCl、0.2 mol·L^-1EDTA、0.8 mol·L^-1HCl以及0.4 mol·L^-1盐酸羟胺,按固液比1:50超声辅助浸提45 min,各组分得率较高,龙须菜中的总硼量高达(125.00±4.10) mg·kg^-1.结果同时表明,龙须菜中约有20.91%的硼以水溶态形式存在,约有55.23%的硼以半束缚态形式存在,束缚态硼占总硼23.86%.实验结果可为龙须菜的食用安全性和硼标准制定提供科学依据.

In order to investigate the effect of boron on food safety of Gracilariopsis lemaneiformis,the Gracilariopsis lemaneiformis from Fujian were chosen as the raw material.Continuous leaching and inductively coupled plasma emission spectroscopy(ICP-OES)were applied to examine the Gracilariopsis lemaneiformis in Fujian.The distribution and speciation of boron was investigated and the extraction conditions were optimized.The optimized extraction parameters were as follows:crushing the asparagus to 20~30 mesh,using 0.2 mol·L^-1 NaCl,0.2 mol·L^-1 EDTA,0.8 mol·L^-1 HCl,and 0.4 mol·L^-1 hydroxylamine hydrochloride,leaching for 45 min in 1:50 solid-liquid ratio,the yield of each component was higher.The total boron content in Gracilariopsis lemaneiformis was(125.00±4.10)mg·kg^-1.The results showed that about 20.91%of the boron in the Gracilariopsis lemaneiformis had a water-soluble form,about 55.23%of the boron existed in a semi-bound state,and about 23.86%of the boron existed in a bound state.The experimental results can provide a scientific basis for the food safety of Gracilariopsis lemaneiformis and the setting of aluminum standards.