改良咽拭子采集法在新冠肺炎患者中的获益研究

Benefit study of improved throat swab collection in patients with novel coronavirus pneumonia

目的探讨改良咽拭子采集法在新型冠状病毒肺炎(COVID-19)患者诊断中的应用效果。方法选取2020年2月9日~4月4日在武汉火神山医院住院的218例轻型和普通型COVID-19确诊病例作为研究对象,通过掷硬币的方式进行随机分组,正面有花的为改良组(122例),反面为对照组(96例)。改良组患者采用改良咽拭子采集法,对照组患者采用传统方法采集咽拭子。使用实时荧光RT-PCR方法检测分析新冠病毒核酸阳性率。结果改良组核酸检测阳性28例,可疑阳性8例,核酸检测总阳性率29.5%。对照组核酸检测阳性15例,可疑阳性1例,核酸检测总阳性率16.7%。改良组患者的核酸检测总阳性率高于对照组,差异有统计学意义(P<0.05)。结论经改良法采集咽拭子与传统方法采集比较,新冠病毒核酸检测阳性率有所提高,可操作性强,患者耐受性好,为COVID-19的早发现、早诊断、早治疗提供依据。

Objective To investigate the application effect of improved throat swab collection in the diagnosis of patients with novel coronavirus pneumonia.Methods A total of 218 patients with mild and common type of novel coronavirus pneumonia in Huoshenshan Hospital of Wuhan City from February 9 to April 4,2020 were randomly divided into two groups by flipping a coin.Those with flowers on the front were the improved group(122 cases),and the reverse side was the control group(96 cases).The improved pharyngeal swab was collected in the improved group,while the traditional method was used in the control group.The novel coronavirus nucleic acid positive rate was detected and analyzed by real-time fluorescence RT-PCR.Results In the improved group,28 cases were positive in nucleic acid detection,8 cases were suspicious,the total positive rate of nucleic acid detection was 29.5%.In the control group,15 cases were positive in nucleic acid detection,1 cases were suspicious,the total positive rate of nucleic acid detection was 16.7%.The total positive rate of nucleic acid detection in the improved group was higher than that in the control group,and the difference was statistically significant(P<0.05).Conclusion The positive rate of nucleic acid detection use the improved method is higher than that use the traditional method.It has highly maneuverable and well patient tolerated.It can provide the basis for early detection,diagnosis and treatment of novel coronavirus pneumonia.