甘露寡糖分离纯化研究进展

Research Progress on Separation and Purification of Mannan Oligosaccharide

甘露寡糖具有润肠通便、降血脂、抗结肠炎症、增强免疫及调节肠道菌群等生理功能,在食品药品等领域具有广阔的应用前景。水解法制得的甘露寡糖含有单糖、未分解聚糖、不同聚合度寡糖及盐离子等杂质,还需要进一步分离纯化。综述了近年来国内外甘露寡糖的主要纯化方法包括柱层析法、膜分离法、乙醇沉淀法和微生物发酵法等。总结了各种方法的原理、应用范围和应用实例,并对不同方法的优缺点进行了分析。

Mannan oligosaccharides have a series of physiological functions such as,lower blood fat,resist colon inflammation,enhance immunity and regulate intestinal flora and have broad application prospects in the fields of food and medicine.The mannan-oligosaccharide products prepared by the hydrolysis method usually contain monosaccharides,unhydrolyzed polysaccharides,oligosaccharides with different polymerization degree,salt ions and other impurities,which need further separation and purification.The main purification methods of mannose oligosaccharides in recent years are reviewed,including column chromatography,membrane separation,ethanol precipitation and microbial fermentation.The principle,application scope and application examples of various methods are summarized,and the advantages and disadvantages of different methods are analyzed.