青海地区胃腺癌患者TGFβI基因的甲基化状态

Methylation of TGFβI gene of patients with gastric adenocarcinoma in Qinghai area

目的通过检测青海地区胃腺癌患者癌组织及癌旁组织中的TGFβI基因启动子区域的甲基化状态,探讨TGFβI基因甲基化与青海地区胃腺癌发生的相关程度。方法收集青海大学附属医院经胃镜及病理首次确诊的28例胃腺癌住院患者为研究对象,采用亚硫酸氢盐测序(BSP)检测各样本TGFβI基因甲基化状态,比较癌组织和癌旁组织的甲基化状态差异。结果﹑胃腺癌组织甲基化克隆个数为478个,其甲基化率为11.42%;癌旁组织甲基化克隆个数为293个,其甲基化率为6.98%;胃腺癌组织甲基化程度高于与其相匹配的癌旁组织,差异具有统计学意义(P<0.001)。结论青海地区患者胃腺癌组织甲基化状态显著高于与其相匹配的癌旁组织甲基化状态,说明青海地区胃腺癌高发可能与TGFβI基因启动子甲基化程度过高有关。

Objective To investigate the methylation of TGFβI gene in the promoter region in cancer tssuesand adjacent tissues of patients with gastric adenocarcinoma in Qinghai area.Methods 28 confirmed cases with gas-tric ademocarcinoma diagnosed by gastroscopy and pathology for the first time in the Affiliated Hospital of QinghaiUniversity were selected as the research objects.The methylation of TCFpI gene was detected by Bisulfite Sequen-cing(BSP)to compare the differences of methylation between cancer tissues and adjacent tissues.Results There were 478 methylated clones in the tissues of gastric adenocarcinoma with an average methylation rate of 11.42%;there were 293 methylated clones in adjacent tissues with an average methylation rate of 6.98%o;the degree of meth-ylation in the tissues of gastric adenocarcinoma was significantly higher than that of its matched adjacent tissues.Thedifference was statistically important(P<0.001).Conclusion The degree of methylation in gastric adenocarcinomawas significantly higher than.that of its matched adjacent tissues,which indicated that the high incidence of gastricadenocarcinoma in Qinghai may be related to the high degree of methylation of TGFβI gene promoter.