基于网络药理学探索茵陈蒿汤治疗乙型病毒性肝炎作用机制研究

Research on the Mechanism of Yinchenhao Decoction in Treating Virus B Hepatitis Based on Network Pharmacology

目的使用网络药理学的研究方法对茵陈蒿汤治疗乙型病毒性肝炎的作用机制进行研究探讨。方法在TCMSP数据库平台筛选茵陈蒿汤的活性化合物成分,运用Perl语言以及uniprot数据库得到茵陈蒿汤的基因靶点;在GeneCards数据库检索和筛选慢性乙型肝炎相关疾病基因靶点进行检索,使用R语言筛选出共同的靶点并构建韦恩图;应用Cytoscape 3.7.1软件构建"活性成分-靶点"网络图;将共同靶点输入STRING数据库,获得蛋白-蛋白相互作用(protein-protein interaction,PPI)网络模型;使用R语言对"活性成分-靶点"相互作用网络图中的核心靶点基因进行GO功能注释和KEGG通路富集分析。结果预测得到茵陈蒿汤活性化合物成分中41种以及潜在靶点101个;得到乙型肝炎疾病相关靶点992个,茵陈蒿汤-乙型病毒性肝炎共同靶点53个,度值较高的活性成分包括β-谷甾醇(β-sitosterol),槲皮素(quercetin),山柰酚(kaempferol)等,度值较高的靶点蛋白包括孕激素受体PGR,RELA原癌基因(p65),雌激素受体1(ESR1),凝血因子Ⅶ(F7),过氧化物酶体增殖物激活受体γ(PPARG)等;PPI网络中的核心基因包括白细胞介素-6(IL-6)、半胱氨酸天冬氨酸蛋白水解酶-3(CASP3)、血管内皮生长因子(VEGFA)、雌激素受体α(ESR1)等;共同作用靶点主要富集于84种生物功能与130条信号通路上。结论通过运用网络药理学的研究方法发现茵陈蒿汤治疗乙型肝炎具有多靶点、多通路的特点,从抗病毒、调节机体免疫、保护肝脏细胞、抑制癌变等多个机制发挥治疗作用。

Objective To investigate the mechanism of Yinchenhao Decoction(YCHD)in the treatment of virus B hepatitis by network pharmacology.Methods The active compounds of YCHD were screened on the platform of TCMSP database,and the genetic targets of YCHD were obtained by using Perl language and uniprot database.GeneCards database was used to search and screen the gene targets of virus B hepatitis.R language was used to screen the common targets and construct the Venn diagram.Cytoscape 3.7.1 software was used to construct the network diagram of"active ingredient-target".The common target was input into the STRING database to obtain the protein-protein interaction(PPI)network model.GO functional annotation and KEGG pathway enrichment analysis were performed for the core target genes in the"active ingredient-target"interaction network diagram by R language.Results 41 active compounds and 101 potential targets were predicted.We get 992 targets related to virus B hepatitis,and 53 common targets of YCHD-virus B hepatitis.And the higher degree of active ingredients includingβ-sitosterol,quercetin,kaempferol,and the higher degree of target proteins including progesterone receptor PGR,RELA(p65),estrogen receptor 1(ESR1),blood coagulation factorⅦ(F7),PPARG,etc.;The core genes in PPI network include interleukin-6(IL-6),cysteine aspartic proteolytic enzyme-3(CASP3),vascular endothelial growth factor(VEGFA),estrogen receptor complex(ESR1),etc.The co-acting targets were mainly concentrated in 84 biological functions and 130 signaling pathways.Conclusion Through the application of network pharmacology,we found that YCHT has the characteristics of multi-target and multi-pathway in the treatment of virus B hepatitis,and plays a therapeutic role in the mechanism of anti-virus,regulating the body immunity,protecting liver cells and inhibiting carcinogenesis.