羧甲基壳聚糖-羧基葡聚糖凝聚微滴的制备及其酵母细胞毒性

Preparation of Coacervate Microdroplets Based on Carboxymethyl Chitosan andCarboxyl Dextran and Its Cytotoxicity Towards Yeast

凝聚微滴通过自组装在细胞表面形成仿生细胞壁,可保护细胞,降低外界刺激和化学环境引起的损害。天然高分子具有生物相容性好及可生物降解等优点,可用于制备结构稳定的凝聚微滴,探索其在细胞保护领域的应用具有重要价值。本实验以壳聚糖和葡聚糖天然高分子为原料,制备羧甲基壳聚糖(CMCS)和羧基葡聚糖(DEX-COOH),进而制备凝聚微滴,利用傅里叶变换红外光谱(FTIR)、紫外-可见吸收光谱(UV-vis)、倒置荧光显微镜(FIM)、激光扫描共聚焦显微镜(CLSM)与动态光散射(DLS)等技术对其进行表征。结果表明,在CMCS与DEX-COOH的质量浓度比为1∶2条件下制备的凝聚微滴的稳定性最好,该凝聚微滴对酿酒酵母未产生细胞毒性,在细胞保护领域具有潜在应用。

Coacervate microdroplets can form an artificial cell wall on the cell surface via self-assembly,which can protect the cell and decrease the damage caused by external stimulation and chemical environment.Natural polymers with advantages such as good biocompatibility and biodegradability,can be employed to prepare coacervate microdroplets with stable structure.It is of great significance to explore the preparation methods of coacervate microdroplets and their application in the cell protection.In this paper,chitosan and dextran were used to prepare the basic element materials of coacervate microdroplets,carboxymethyl chitosan(CMCS)and carboxyl dextran(DEX-COOH),which were employed to construct the coacervate microdroplets.Some techniques such as Fourier Transform Infrared Spectrometer(FTIR),UV-visable spectrophotometry(UV-vis),fluorescent inverted microscope(FIM),confocal laser scanning microscope(CLSM)and dynamic light scattering(DLS)were used to characterize the structure of coacervate microdroplets.The results showed that the stability of the coacervate microdroplets prepard under the condition that the mass concentration ratio of CMCS and DEX-COOH was 1∶2.Without cytotoxicity to saccharomyces cerevisiae,the prepared coacervate microdroplets present potential applications in the improvement of probiotics resistance to external stimulation.