莪术联合放疗对宫颈腺癌Hela细胞凋亡的影响

Effect of zedoary turmeric combined with radiotherapy on apoptosis of cervical adenocarcinoma HeLa cells

目的采用体外培养宫颈腺癌Hela细胞,观察莪术联合放疗对宫颈腺癌Hela细胞增殖、凋亡的影响。方法采用CCK-8法检测不同浓度莪术油注射液对Hela细胞生长的抑制作用。设立空白对照组、单药组、6Gy照射组、10Gy照射组、药物+6Gy照射组、药物+10Gy照射组,CCK-8法检测莪术油联合放疗对Hela细胞的抑制作用,流式细胞术检测莪术油联合放疗对Hela细胞凋亡的影响。结果莪术油作用后,Hela细胞生长受到抑制,且细胞抑制率与药物浓度显著相关(P<0.05)。CCK-8法检测显示,与空白对照组相比,单药组、6Gy照射组、10Gy照射组、药物+6Gy照射组、药物+10Gy照射组细胞生长均受到抑制(P<0.05)。与6Gy照射组相比,药物+6Gy照射组细胞抑制率升高(P<0.05);与10Gy照射组相比,药物+10Gy照射组细胞抑制率升高(P<0.05)。流式细胞术显示,与6Gy照射组相比,药物+6Gy照射组细胞凋亡率升高(P<0.05);与10Gy照射组相比,药物+10Gy照射组细胞凋亡率升高(P<0.05)。结论莪术油可抑制宫颈癌Hela细胞增殖,莪术油联合放疗可使Hela细胞抑制率和凋亡率显著升高,莪术油可增强Hela细胞放射敏感性。

Objective In this study,HeLa cells were cultured in vitro,and the effects of zedoary turmeric combined with radiotherapy on the proliferation and apoptosis of HeLa cells were observed.The inhibitory effect of zedoary turmeric oil injection on the growth of HeLa cells was detected by CCK-8 method.Methods Blank control group,single drug group,6 Gy irradiation group,10 Gy irradiation group,drug+6 Gy irradiation group and drug+10 Gy irradiation group were established.The inhibitory effect of zedoary turmeric oil combined with radiotherapy on HeLa cells was detected by CCK-8 method,and the apoptosis of HeLa cells was detected by flow cytometry.Results The results showed that the growth of HeLa cells was inhibited by zedoary turmeric oil,and the inhibition rate was significantly correlated with drug concentration(P<0.05).CCK-8 assay showed that compared with the blank control group,the growth of cells in single drug group,6 Gy irradiation group,10 Gy irradiation group,drug+6 Gy irradiation group and drug+10 Gy irradiation group were all inhibited(P<0.05).Compared with the 6 Gy irradiation group,the cell inhibition rate of the drug+6 Gy irradiation group increased(P<0.05);compared with the 10 Gy irradiation group,the cell inhibition rate of the drug+10 Gy irradiation group increased(P<0.05).The results of flow cytometry showed that compared with 6 Gy irradiation group,the apoptosis rate of drug+6 Gy irradiation group was increased(P<0.05);compared with 10 Gy irradiation group,the apoptosis rate of drug+10 Gy irradiation group was increased(P<0.05).Conclusion It is concluded that zedoary turmeric oil can inhibit the proliferation of HeLa cells,zedoary turmeric oil combined with radiotherapy can significantly increase the inhibition rate and apoptosis rate of HeLa cells,and zedoary turmeric oil can enhance the radiosensitivity of HeLa cells.