甘肃省孢囊线虫新记录种:艾球孢囊线虫

Globodera artemisiae,a new cyst nematode record in Gansu,China

在2018−2019年,在甘肃省天祝县高寒草地艾蒿(Artemisia argyi)根际分离得到1个孢囊线虫群体,采用形态学结合分子生物学技术进行了种类鉴定。该孢囊线虫群体形态特征:孢囊呈褐色,球形,颈部较为突出,无阴门锥;阴门膜孔为环膜孔型,无下桥和泡状突。二龄幼虫线形,头部与体驱间缢缩不明显,口针强壮。尾部圆锥形,末端较尖锐,经形态学初步鉴定为艾球孢囊线虫(Globodera artemisiae)。对该群体ITS-rDNA和28S-rRNA D2-D3区进行扩增,分别得到948 bp和1015 bp两个片段。测序后进行聚类分析,发现该群体与艾球孢囊线虫聚为一支,置信度为97%以上。结合形态学和分子生物学特征,将该群体鉴定为艾球孢囊线虫,这也是甘肃首次报道球孢囊线虫属线虫,系甘肃省新记录种。

In 2018−2019,a cyst nematode population was isolated from the rhizosphere of Artemisia argyi on an alpine grassland in Tianzhu County,Gansu Province,China.The morphological characteristics of this nematode population were:brown spherical cysts,a prominent neck,generally circumfenestrate,with no vulval cone,underbridge,or bullae.Secondstage juveniles were vermiform,with inconspicuous head constriction,and a strong stylet.The tail was conical and sharply pointed.The ITS-rDNA and 28S-rRNA D2-D3 regions of the population were amplified,from which 948 and 1015 bp fragments were obtained,respectively.After sequencing,cluster analysis showed that the population was clustered as Globodera artemisiae,with a confidence of over 97%.This population was identified as G.artemisiae based on its morphological and molecular characteristics.This is the first report of the genus Globodera and a new species record in Gansu Province.