胃癌组织中幽门螺旋杆菌感染与原癌基因表达、病理特征的关系及其对术后癌复发的评估价值

The relationship between Helicobacter pylori infection in gastric cancer and proto-oncogene expression,pathological features and its value in evaluating postoperative recurrence

目的研究胃癌组织中幽门螺旋杆菌(HP)感染与原癌基因表达、病理特征的关系及其对术后癌复发的评估价值。方法选择2016年3月-2018年3月期间在西安市第一医院接受手术切除的胃癌患者46例作为胃癌组,同期接受胃镜活检并证实为正常胃黏膜的患者35例作为对照组,检测胃癌组织及正常胃黏膜组织中HP感染率及原癌基因β-catenin、cyclinD1、c-myc的mRNA表达量,随访胃癌患者术后1年的复发率及生存率。结果胃癌组的胃癌组织中HP感染率及β-catenin、cyclinD1、c-myc表达量均明显高于对照组,差异有统计学意义(P<0.05),且HP感染的胃癌组织中β-catenin、cyclinD1、c-myc的表达量明显高于HP未感染的胃癌组织,差异有统计学意义(P<0.05)。低未分化、T3~T4、有淋巴结浸润的胃癌组织的HP感染率明显高于中高分化、T1~T2、无淋巴结浸润的胃癌组织,差异有统计学意义(P<0.05)。HP感染胃癌患者的术后1年复发率高于HP未感染胃癌患者,术后1年生存率低于HP未感染胃癌患者,但差异无统计学意义(P>0.05)。结论胃癌患者HP感染与原癌基因表达增加、病理特征恶化有关,是否影响远期预后仍需进一步随访。

Objective To study the relationship between Helicobacter pylori(HP) infection in gastric cancer and protooncogene expression,pathological features and its value in evaluating postoperative recurrence.Methods Gastric cancer patients who underwent surgical resection in our hospital from March 2016 to March 2018 were selected as the gastric cancer group(n=46);patients who underwent gastroscopic biopsy and were confirmed as normal gastric mucosa during the same time were selected as control group(n=35).HP infection rate and the expression of proto-oncogene β-catenin,cyclinDl and c-myc in gastric cancer tissues and normal gastric mucosa tissues were detected.The recurrence rate and survival rate of gastric cancer patients were followed up for 1 year.Results The HP infection rate and the expression of β-catenin,cyclinDl and c-myc in gastric cancer tissues were significantly higher than those of normal gastric mucosa tissues(P<0.05).The expression of β-catenin,cyclinDl and c-myc in HP infected gastric cancer were significantly higher than those in nonHP-infected gastric cancer(P<0.05).The HP infection rate in low undifferentiated,T3-T4,lymph node-infiltrated gastric cancer tissues were significantly higher than those in moderately well-differentiated,T1-T2 and non-lymph node-infiltrated gastric cancer tissues(P<0.05).The 1-year recurrence rate of HP-infected gastric cancer patients was higher than that of nonHP-infected gastric cancer patients,and the 1-year survival rate was lower than that of non-HP-infected gastric cancer patients,but the differences had no statistical significance(P>0.05).Conclusions HP infection in gastric cancer patients relate with increased expression of oncogene and deterioration of pathological features.Whether HP infection affects longterm prognosis needs further follow-up.