AHSA1基因缺失酿酒酵母株的构建及其对生长的影响

Construction of AHSA1 gene deletion strain of Saccharomyces cerevisiae and its effect on yeast growth

目的构建酿酒酵母AHSA1基因缺失菌株,并观察其对生长的影响。方法运用基因同源重组技术构建单基因缺失的酿酒酵母株,采用生长曲线测定法和克隆形成实验检测AHSA1缺失对生长的影响。结果成功构建AHSA1基因缺失酵母菌株。与野生型酵母相比,AHSA1缺失菌株的克隆形成能力及短期增殖活性明显降低。结论AHSA1基因缺失可抑制酿酒酵母生长。

Objective To construct AHSA1 gene deletion strain of Saccharomyces cerevisiae and detect its effect on yeast growth.Methods AHSA1 gene deletion strain of S.cerevisiae was constructed using homologous recombination technique.Yeast growth was detected by growth curve and clone formation assay.Results AHSA1 gene deletion strain was successfully constructed.The clone formation and short-term proliferative activity were lower in AHSA1 gene deletion strain than in wild type yeast.Conclusion AHSA1 gene deletion inhibits the growth of Saccharomyces cerevisiae.