甲基苯丙胺对大鼠心脏血管通透性的影响初探

Effect of methamphetamine on cardiovascular permeability in rats: a preliminary study

目的研究甲基苯丙胺(METH)对大鼠心肌组织病理学改变及心血管通透性的影响。方法建立METH亚急性、慢性、复吸组SD大鼠中毒模型;分离、培养原代大鼠血管内皮细胞,用浓度0.5 mmol/L METH处理0.5、2、4、12、24 h。HE染色观察心肌病理变化,ELISA检测血清心肌肌钙蛋白I(cTnI)含量,蛋白印迹检测心肌和VEC血管内皮生长因子(VEGF)表达。结果慢性组及复吸组大鼠心脏出现多灶性甚至弥漫性心肌间质出血;复吸组cTnI水平较对照组明显升高(P<0.01);亚急性组较对照组心肌VEGF表达明显升高(P<0.01)。METH处理0.5 h心肌内皮细胞VEGF表达即升高,24 h达到峰值(P<0.01)。结论METH可诱导大鼠心血管内皮细胞VEGF表达上调,进而引起心血管通透性升高。

Objective To investigate the effect of methamphetamine(METH)on myocardial pathology and cardiovascular permeability in rats.Methods Subacute,chronic and relapsing METH models were established in SD rats.Primary rat vascular endothelial cells(VECs)were isolated and cultured,and then treated with 0.5 mmol/L METH for 0.5,2,4,12,24 h.Cardiac pathology,serum cardiac troponin I(cTnI)level,and vascular endothelial growth factor(VEGF)expression of myocardium and VECs were determined by HE stain,ELISA and Western blot,respectively.Results Multifocal or diffuse myocardial hemorrhage was observed in chronic and relapsing group.Serum cTnI content was higher in relapsing group(P<0.01),while cardiac VEGF expression was increased in subacute group(P<0.01)compared with control group.METH upregulated VEGF expression of VECs after 0.5 h,with the top at 24 h(P<0.01).Conclusion METH induces VEGF overexpression of cardiovascular endothelial cells in rats,thus promoting the cardiovascular permeability.