红景天苷调节创伤后应激障碍大鼠海马神经元自噬的研究

A study of salidroside regulating autophagy level in hippocampal neurons of rats with post-traumatic stress disorder

目的探究红景天苷对创伤后应激障碍(post-traumatic stress disorder,PTSD)大鼠空间记忆、学习能力、恐惧的影响及可能机制。方法采用国际上认可的单一延长刺激(single prolonged stress,SPS)方法建立PTSD大鼠模型,应用Morris水迷宫实验观察红景天苷对SPS大鼠空间学习记忆能力的影响,使用旷场实验观察红景天苷对SPS大鼠恐惧的影响,采用免疫印迹法及免疫荧光方法检测海马神经元mTOR、TEFB、LC3、PGC-1α的蛋白水平和免疫反应性。结果SPS+红景天苷组大鼠空间记忆能力较SPS组大鼠有明显改善,焦虑水平有所下降。免疫荧光染色显示了SPS大鼠海马神经元TFEB、LC3、mTOR、PGC-1α的改变,Western blot检测显示SPS大鼠海马神经元LC3、PGC-1α水平上升(P<0.05),SPS+红景天苷组与SPS组相比,PGC-1α、LC3显著降低(P<0.05)。结论红景天苷可能通过调节SPS大鼠海马神经元细胞自噬途径影响大鼠的焦虑行为及空间记忆能力。

Objective To investigate the effect of salidroside on spatial memory,learning and fear in rats of PTSD and the probably involved mechanisms.Methods Establishment of a PTSD rat model using an internationally recognized SPS method,and Morris water mase behavioural test was used for the spatial memory ability of rats.Evaluating the fear of rats in open field test.Immunofluorescence and Western blot were used to examine microtubes-related proteins expression.Results The spatial memory and anxiety were made better in SPS+salidroside group than in SPS group.The expression levels of TFEB、LC3、mTOR and PGC-1αin hippocampal neurons were lower in SPS+salidroside group than in SPS group.Conclusion Salidroside may affect the anxiety behavior and spatial memory ability of rats by regulating the autophagy pathway of hippocampal neurons in SPS rats.