颈动脉窦压力感受器电刺激对肥胖相关性高血压大鼠的影响

Effects of Carotid Baroreceptor Stimulation on Obesity-related Hypertensive Rats

目的探讨颈动脉窦压力感受器电刺激(carotid baroreceptor stimulation,CBS)对肥胖相关性高血压大鼠的影响。方法20只雄性SD大鼠随机分为4组,即正常对照(C-sham)组、肥胖高血压(OH-sham)组及CBS干预的两个组(C-CBS、OH-CBS组)。采用高脂饮食和10%果糖水喂养4周建立肥胖相关性高血压模型。所有大鼠均进行CBS仪器植入术,OH-CBS与C-CBS组仪器发放电刺激,OH-sham和C-sham组仪器不发放刺激。对所有大鼠每周进行血压及体质量的测量,12周后,测量空腹血糖、24h尿钠、血清尿素、肌酐、尿酸、胰岛素水平,记录体表心电图及内脏脂肪称重。结果与C-sham组比较,OH-sham组的血压、体质量、内脏脂肪含量、空腹血糖、血清胰岛素水平、尿酸水平均显著升高(P<0.05),心率呈升高趋势,24h尿钠含量显著降低(P<0.05);CBS刺激可明显降低肥胖高血压组的血压、心率、体质量、内脏脂肪含量、空腹血糖、血清胰岛素水平,同时显著提高24h尿钠含量(P<0.05),但对尿酸水平有升高趋势;CBS刺激对正常对照组的血压、心率、体质量、内脏脂肪含量、空腹血糖、24h尿钠含量均无明显影响,但明显降低血清胰岛素水平(P<0.05),对尿酸水平有升高趋势。结论CBS通过降低交感神经张力对肥胖相关性高血压大鼠有明显的积极作用。

Objective To explore the effect of carotid baroreceptor stimulation(CBS)on obesity-related hypertensive rats.Methods Twenty male rats(Sprague-Dawley)were randomized into 4 groups:normal control(C-sham)group,obesity-hypertension(OH-sham)group and the two groups(C-CBS,OH-CBS group)with CBS intervention.An obesity-related hypertension model was established by feeding with high-fat diet and 10%fructose water.Carotid baroreceptor stimulators were implanted at the same time for all rats.The CBS instruments in the OH-CBS and C-CBS groups worked normally,but in the OH-sham and C-sham groups did not work.Blood pressure and body weight were measured every week for all rats.After 12 weeks,rats were sacrificed.Fasting blood glucose,24 h urinary sodium,serum urea,creatinine,uric acid,insulin levels were tested,ECG were recorded and visceral fat were weighed.Results Compared with the control(C-sham)group,the blood pressure,body weight,visceral fat weight,fasting blood glucose,serum insulin levels,and uric acid levels of the obese hypertension(OH-sham)group were significantly increased(P<0.05),and the heart rate also presented increasing trend,meanwhile 24 h urinary sodium were significantly reduced(P<0.05).CBS stimulation can significantly reduce blood pressure,heart rate,body weight,visceral fat weight,fasting blood glucose and serum insulin levels in the obese hypertension group,and also significantly increase 24 h urinary sodium(P<0.05),but there was a tendency of increasing uric acid levels.CBS stimulation had no significant effect on blood pressure,heart rate,body weight,visceral fat weight,fasting blood glucose,and 24 h urinary sodium in the normal control group,but significantly reduced serum insulin levels(P<0.05)and had a tendency to increase the uric acid level.Conclusion CBS has a significant positive effect on obesity-related hypertensive rats by reducing sympathetic tone.