NRG1α和NRG1β在人肾母细胞瘤中表达的差异研究

Comparison of Expression of NRG1αand NRG1βin Human Nephroblastoma

目的:探究肾母细胞瘤(Wilms氏瘤,WT)中,类表皮生长因子(EGF)家族成员神经调节蛋白-1(NRG1)的表达和分布情况。方法:采用免疫荧光染色法检测来自20位不同肿瘤级别患者WT组织中NRG1α和NRG1β两个亚型的表达,以0~4分为基础进行免疫染色强度评估。结果:NRG1α与NRG1β均表达于WT肿瘤组织中,且NRG1α表达水平(3.5±0.61)高于NRG1β(2.85±0.59)(P<0.05)。结论:NRG1两种亚型在WT发生发展中均可能发挥作用。NRG1α在WT早期检测上更具应用前景,而定位于细胞膜上的NRG1β可能更能特异地提示肿瘤恶性程度。

Objective:To investigate the expression and distribution of the two variants(αandβ)of neuregulin-1(NRG1),an epidermal growth factor(EGF)family member,in different stages of Wilms′tumor(WT).Methods:Immunofluorescence staining was used to detect the expression of NRG1αand NRG1βin cell nucleus,cytoplasma and cell membrane among 20 human WT tissues with different stages.The intensity of immunostaining was evaluated on 0 to 4 point standard.Results:Both subtypes of NRG1 were expressed in WT,with NRG1α(3.5±0.61)more highly expressed and widely distributed than NRG1β(2.85±0.59)(P<0.05).Conclusion:Both variants of NRG1 play important roles in the development of WT.NRG1αmay serve as a potential promising biomarker in the early detection of WT,while NRG1βlocated on the cell membrane may more specifically indicate tumor malignancy.