摘要
OBJECTIVE:To establish an in vitro model of radiation-induced lung injury using rat lung alveolar macrophages(NR8383).METHODS:Using a medical electronic linear accelerator,cells were irradiated with either 0 Gy or 6 Gy X-rays.At 6,12,24,30 and 48 h,the DNA damage index(8-OHd G)and lipid damage index(MDA)were measured in the two groups.We also determined the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6)and transforming growth factor-β(TGF-β).RESULTS:The levels of 8-OHd G and MDA in the 6 Gy irradiation group were higher than those in the0 Gy group at 6,12,24,30 and 48 h after irradiation.The levels reached the highest value-6 h after irradiation,and then gradually decreased.The levels of the inflammatory factors TNF-α,TGF-βand IL-6 were higher in the 6 Gy irradiation group than those in the 0 Gy group at 6,12,24,30 and 48 h after irradiation.CONCLUSION:Six Gy X-ray irradiated NR8383 cells can be used to establish an in-vitro model of radiation-induced lung injury.The levels of 8-OHd G,MDA,TNF-α,TGF-βand IL-6 can be used as effective evaluation indicators.
基金
Supported by the National Natural Science Foundation of China the Mechanism Underlying Nourishing Yin,Clearing Lung and Activating Blood Circulation to Regulate AMPK Mediated Autophagy in Radiation-induced Lung Injury(No.81673754)。
