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Lichong decoction inhibits micro-angiogenesis by reducing the expressions of hypoxia inducible factor-1αand vascular endothelial growth factor in hysteromyoma mouse model 被引量:8

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摘要 OBJECTIVE:To investigate the efficacy of Lichong decoction(LD)from Traditional Chinese Medicine,on micro-angiogenesis in a mouse model of hysteromyoma.METHODS:A mouse model of hysteromyoma was developed by orthotopic intrauterine injection of primary human myoma cells isolated from patients from the Beijing Obstetrics and Gynecology Hospital into CB-17 Scid mice.Mice were administered high-dose LD,low-dose LD,mifepristone or water(control)daily by gavage for 4 weeks.Uterine diameter and coefficient(uterine weight/body weight)were measured.Uterine morphology was assessed by light microscopy(hematoxylin and eosin)and transmission electron microscopy.Serum levels of estradiol,progesterone,follicle-stimulating hormone and luteinizing hormone(LH)were measured by enzyme-linked immunosorbent assay.Uterine protein expression of hypoxia inducible factor(HIF)-1α,CD31 and proliferating cell nuclear antigen(PCNA)was detected by immunohistochemistry.VEGF and HIF-1αm RNAs were quantified by RT-PCR.RESULTS:High-dose LD,low-dose LD and mifepristone reduced uterine diameter and coefficient,and attenuated the morphologic abnormalities associated with hysteromyoma.High-dose LD,low-dose LD and mifepristone inhibited hysteromyoma-induced micro-angiogenesis,as evidenced by a decrease in the number of new microvessels co-immunostaining for CD31 and PCNA(P<0.01).High-dose LD and mifepristone lowered serum levels of estradiol,progesterone and LH(P<0.05).High-dose LD,low-dose LD and mifepristone down-regulated HIF-1αm RNA and protein expressions and VEGF m RNA expression(P<0.01).CONCLUSION:The inhibition of hysteromyoma by LD may involve reductions in HIF-1αand VEGF expression and suppression of micro-angiogenesis.
出处 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2020年第6期928-937,共10页 中医杂志(英文版)
基金 Supported by the National Natural Science Foundation of China(to Study the Effect and Molecular Mechanism of Lichong Decoction in Inhibiting Immune Escape of Hysteromyoma Through IDO Mediated Immune Editing Pathway,No.81774072 Based on Angiogenesis Microenvironment to Explore the Mechanism of Lichong Decoction in Inhibiting Hysteromyoma by Regulating Ang/Tie-2 Signal Transduction Pathway,No.81373812 Objective to Explore the Regulatory Mechanism of Extracellular Matrix Metabolism of Fuzheng Quyu Traditional Chinese Medicine on the Inhibition of Hysteromyoma,No.81073096) Beijing Municipal Natural Science Foundation(Effect of Lichong Decoction on Balance Drift of Angiogenesis/Inhibitor of Hysteromyoma Based on Lnc RNA Signal Pathway,7202015) Inheritance and Innovation of Traditional Chinese Medicine"Hundred,Thousand and Ten Thousand"Talent Project(Qihuang Project)the Fourth Batch of National Research and Training Project of Chinese Medicine Talents(TCM Education And Development 2017 No.24)。
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